PUBLICATION
Super-Resolution Imaging Reveals the Nanoscale Distributions of Dystroglycan and Integrin Itga7 in Zebrafish Muscle Fibers
- Authors
- Shivanna, K., Astumian, M., Raut, P., Ngo, V.N., Hess, S.T., Henry, C.
- ID
- ZDB-PUB-230730-49
- Date
- 2023
- Source
- Biomedicines 11(7): (Journal)
- Registered Authors
- Henry, Clarissa A.
- Keywords
- Danio rerio, Dystroglycan, FPALM, fluorescence, integrins, localization microscopy, muscle fibers, muscular dystrophy, super-resolution, zebrafish
- MeSH Terms
- none
- PubMed
- 37509580 Full text @ Biomedicines
Citation
Shivanna, K., Astumian, M., Raut, P., Ngo, V.N., Hess, S.T., Henry, C. (2023) Super-Resolution Imaging Reveals the Nanoscale Distributions of Dystroglycan and Integrin Itga7 in Zebrafish Muscle Fibers. Biomedicines. 11(7):.
Abstract
Cell signaling is determined partially by the localization and abundance of proteins. Dystroglycan and integrin are both transmembrane receptors that connect the cytoskeleton inside muscle cells to the extracellular matrix outside muscle cells, maintaining proper adhesion and function of muscle. The position and abundance of Dystroglycan relative to integrins is thought to be important for muscle adhesion and function. The subcellular localization and quantification of these receptor proteins can be determined at the nanometer scale by FPALM super-resolution microscopy. We used FPALM to determine localizations of Dystroglycan and integrin proteins in muscle fibers of intact zebrafish (Danio rerio). Results were consistent with confocal imaging data, but illuminate further details at the nanoscale and show the feasibility of using FPALM to quantify interactions of two proteins in a whole organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping