PUBLICATION
Altered glucocorticoid reactivity and behavioral phenotype in rx3-/- larval zebrafish
- Authors
- Herget, U., Ryu, S., De Marco, R.J.
- ID
- ZDB-PUB-230724-44
- Date
- 2023
- Source
- Frontiers in endocrinology 14: 11873271187327 (Journal)
- Registered Authors
- Herget, Ulrich, Ryu, Soojin
- Keywords
- HPA axis, cortisol, glucocorticoid, pituitary, rx3, stress, zebrafish
- MeSH Terms
-
- Animals
- Glucocorticoids/metabolism
- Hydrocortisone/metabolism
- Larva/metabolism
- Phenotype
- Zebrafish*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 37484970 Full text @ Front Endocrinol (Lausanne)
Citation
Herget, U., Ryu, S., De Marco, R.J. (2023) Altered glucocorticoid reactivity and behavioral phenotype in rx3-/- larval zebrafish. Frontiers in endocrinology. 14:11873271187327.
Abstract
Introduction The transcription factor rx3 is important for the formation of the pituitary and parts of the hypothalamus. Mutant animals lacking rx3 function have been well characterized in developmental studies, but relatively little is known about their behavioral phenotypes.
Methods We used cell type staining to reveal differences in stress axis architecture, and performed cortisol measurements and behavior analysis to study both hormonal and behavioral stress responses in rx3 mutants.
Results and discussion Consistent with the role of rx3 in hypothalamus and pituitary development, we show a distinct loss of corticotrope cells involved in stress regulation, severe reduction of pituitary innervation by hypothalamic cells, and lack of stress-induced cortisol release in rx3 mutants. Interestingly, despite these deficits, we report that rx3-/- larval zebrafish can still display nominal behavioral responses to both stressful and non-stressful stimuli. However, unlike wildtypes, mutants lacking proper pituitary-interrenal function do not show enhanced behavioral performance under moderate stress level, supporting the view that corticotroph cells are not required for behavioral responses to some types of stressful stimuli but modulate subtle behavioral adjustments under moderate stress.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping