PUBLICATION
Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle
- Authors
- Henderson, T.D., Choi, J., Leonard, S.W., Head, B., Tanguay, R.L., Barton, C.L., Traber, M.G.
- ID
- ZDB-PUB-230628-55
- Date
- 2023
- Source
- Antioxidants (Basel, Switzerland) 12(6): (Journal)
- Registered Authors
- Barton, Carrie, Tanguay, Robyn L.
- Keywords
- Danio rerio, lipid peroxidation, metabolomics, sarcopenia, α-tocopherol
- MeSH Terms
- none
- PubMed
- 37371890 Full text @ Antioxidants (Basel)
Citation
Henderson, T.D., Choi, J., Leonard, S.W., Head, B., Tanguay, R.L., Barton, C.L., Traber, M.G. (2023) Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle. Antioxidants (Basel, Switzerland). 12(6):.
Abstract
Muscle wasting occurs with aging and may be a result of oxidative stress damage and potentially inadequate protection by lipophilic antioxidants, such as vitamin E. Previous studies have shown muscular abnormalities and behavioral defects in vitamin E-deficient adult zebrafish. To test the hypothesis that there is an interaction between muscle degeneration caused by aging and oxidative damage caused by vitamin E deficiency, we evaluated long-term vitamin E deficiency in the skeletal muscle of aging zebrafish using metabolomics. Zebrafish (55 days old) were fed E+ and E- diets for 12 or 18 months. Then, skeletal muscle samples were analyzed using UPLC-MS/MS. Data were analyzed to highlight metabolite and pathway changes seen with either aging or vitamin E status or both. We found that aging altered purines, various amino acids, and DHA-containing phospholipids. Vitamin E deficiency at 18 months was associated with changes in amino acid metabolism, specifically tryptophan pathways, systemic changes in the regulation of purine metabolism, and DHA-containing phospholipids. In sum, while both aging and induced vitamin E deficiency did have some overlap in altered and potentially dysregulated metabolic pathways, each factor also presented unique alterations, which require further study with more confirmatory approaches.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping