PUBLICATION
Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish
- Authors
- Chong-Morrison, V., Mayes, S., Simões, F.C., Senanayake, U., Carroll, D.S., Riley, P.R., Wilson, S.W., Sauka-Spengler, T.
- ID
- ZDB-PUB-230628-40
- Date
- 2023
- Source
- Biology Open 12(6): (Journal)
- Registered Authors
- Chong-Morrison, Vanessa, Sauka-Spengler, Tatjana, Wilson, Steve
- Keywords
- Ac/Ds, Antisense, CRISPRi, Enhancer, Zebrafish
- MeSH Terms
-
- Animals
- Clustered Regularly Interspaced Short Palindromic Repeats*
- Epigenome
- Gene Editing
- Zebrafish*/genetics
- PubMed
- 37367831 Full text @ Biol. Open
Citation
Chong-Morrison, V., Mayes, S., Simões, F.C., Senanayake, U., Carroll, D.S., Riley, P.R., Wilson, S.W., Sauka-Spengler, T. (2023) Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish. Biology Open. 12(6):.
Abstract
Due to its genetic amenability coupled with advances in genome editing, zebrafish is an excellent model to examine the function of (epi)genomic elements. Here, we repurposed the Ac/Ds maize transposition system to efficiently characterise zebrafish cis-regulated elements, also known as enhancers, in F0-microinjected embryos. We further used the system to stably express guide RNAs enabling CRISPR/dCas9-interference (CRISPRi) perturbation of enhancers without disrupting the underlying genetic sequence. In addition, we probed the phenomenon of antisense transcription at two neural crest gene loci. Our study highlights the utility of Ac/Ds transposition as a new tool for transient epigenome modulation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping