PUBLICATION
Acute exposure of Isopyrazam damages the developed cardiovascular system of zebrafish (Danio rerio)
- Authors
- Yan, Y., Liang, S., Zhang, T., Deng, C., Li, H., Zhang, D., Lei, D., Wang, G.
- ID
- ZDB-PUB-230411-43
- Date
- 2023
- Source
- Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes 58(4): 367-377 (Journal)
- Registered Authors
- Lei, Daoxi, Wang, Guixue
- Keywords
- Isopyrazam, cardiovascular, oxidative stress, succinate dehydrogenase inhibitor fungicides, toxicity, zebrafish
- MeSH Terms
-
- Animals
- Cardiovascular System*/chemistry
- Embryo, Nonmammalian
- Fungicides, Industrial*/toxicity
- Pyrazoles/toxicity
- Water Pollutants, Chemical*/analysis
- Zebrafish
- PubMed
- 37032599 Full text @ J. Environ. Sci. Health B
Citation
Yan, Y., Liang, S., Zhang, T., Deng, C., Li, H., Zhang, D., Lei, D., Wang, G. (2023) Acute exposure of Isopyrazam damages the developed cardiovascular system of zebrafish (Danio rerio). Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes. 58(4):367-377.
Abstract
Isopyrazam (IPZ) is one of the broad-spectrum succinate dehydrogenase inhibitor fungicides (SDHIs). Although the potential bio-toxicity of SDHIs has been reported hourly, the specific effects focused on the cardiovascular system have remained unclear and piecemeal. Thus, we chose IPZ as a representative to observe the cardiovascular toxicity of SDHIs in zebrafish. Two types of transgenic zebrafish, Tg (cmlc2:GFP) and Tg (flk1:GFP) were used in this study. Healthy embryos at 6 hpf were exposed to IPZ solutions. The statistical data including survival rate, hatching rate, malformed rate, and morphological and functional parameters of the cardiovascular system at 48 hpf and 72 hpf demonstrated that IPZ could cause abnormalities and cardiovascular defects such as spinal curvature, dysmotility, pericardial edema, pericardial hemorrhage, and slowed heart rate, etc. At the same time, the activity of enzymes related to oxidative stress was altered with IPZ. Our results revealed that IPZ-induced cardiovascular toxicity and oxidative stress might be one of the underlying toxic mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping