PUBLICATION
Electric field-guided collective motility initiation of large epidermal cell groups
- Authors
- Sun, Y., Reid, B., Zhang, Y., Zhu, K., Ferreira, F., Estrada, A., Sun, Y., Draper, B., Yue, H., Copos, C., Lin, F., Bernadskaya, Y., Zhao, M., Mogilner, A.
- ID
- ZDB-PUB-230330-57
- Date
- 2023
- Source
- Molecular biology of the cell 34(5): ar48 (Journal)
- Registered Authors
- Draper, Bruce
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Movement
- Phosphatidylinositol 3-Kinases*
- Zebrafish*
- PubMed
- 36989037 Full text @ Mol. Biol. Cell
Citation
Sun, Y., Reid, B., Zhang, Y., Zhu, K., Ferreira, F., Estrada, A., Sun, Y., Draper, B., Yue, H., Copos, C., Lin, F., Bernadskaya, Y., Zhao, M., Mogilner, A. (2023) Electric field-guided collective motility initiation of large epidermal cell groups. Molecular biology of the cell. 34(5):ar48.
Abstract
Recent research has elucidated mechanochemical pathways of single cell polarization, but much less is known about collective motility initiation in adhesive cell groups. We used galvanotactic assays of zebrafish keratocyte cell groups, pharmacological perturbations, electric field switches, particle imaging velocimetry and cell tracking to show that large cell groups initiate motility in minutes towards the cathode. Interestingly, while PI3K-inhibited single cells are biased towards the anode, inhibiting PI3K does not affect the cathode-directed cell group migration. We observed that control groups had fastest cathode-migrating cell at the front, while the front cells in PI3K-inhibited groups were the slowest. Both control and PI3K-inhibited groups rapidly repolarized when the electric field direction was reversed, and the group migration continued after the electric field was switched off. Inhibiting myosin disrupted cohesiveness of keratocyte groups and abolished the collective directionality and ability to switch direction when the electric field is reversed. Our data are consistent with a model according to which cells in the group sense the electric field individually and mechanical integration of the cells results in coherent group motility. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping