PUBLICATION
Origin and diversification of fibroblasts from the sclerotome in zebrafish
- Authors
- Ma, R.C., Kocha, K.M., Méndez-Olivos, E.E., Ruel, T.D., Huang, P.
- ID
- ZDB-PUB-230319-35
- Date
- 2023
- Source
- Developmental Biology 498: 35-48 (Journal)
- Registered Authors
- Huang, Peng, Kocha, Katrinka, Ma, Roger
- Keywords
- Axial skeleton, Collagen, Fibroblasts, Fin mesenchymal cells, Sclerotome, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation
- Cell Lineage
- Fibroblasts
- Somites*
- Zebrafish*
- PubMed
- 36933633 Full text @ Dev. Biol.
Citation
Ma, R.C., Kocha, K.M., Méndez-Olivos, E.E., Ruel, T.D., Huang, P. (2023) Origin and diversification of fibroblasts from the sclerotome in zebrafish. Developmental Biology. 498:35-48.
Abstract
Fibroblasts play an important role in maintaining tissue integrity by secreting components of the extracellular matrix and initiating response to injury. Although the function of fibroblasts has been extensively studied in adults, the embryonic origin and diversification of different fibroblast subtypes during development remain largely unexplored. Using zebrafish as a model, we show that the sclerotome, a sub-compartment of the somite, is the embryonic source of multiple fibroblast subtypes including tenocytes (tendon fibroblasts), blood vessel associated fibroblasts, fin mesenchymal cells, and interstitial fibroblasts. High-resolution imaging shows that different fibroblast subtypes occupy unique anatomical locations with distinct morphologies. Long-term Cre-mediated lineage tracing reveals that the sclerotome also contributes to cells closely associated with the axial skeleton. Ablation of sclerotome progenitors results in extensive skeletal defects. Using photoconversion-based cell lineage analysis, we find that sclerotome progenitors at different dorsal-ventral and anterior-posterior positions display distinct differentiation potentials. Single-cell clonal analysis combined with in vivo imaging suggests that the sclerotome mostly contains unipotent and bipotent progenitors prior to cell migration, and the fate of their daughter cells is biased by their migration paths and relative positions. Together, our work demonstrates that the sclerotome is the embryonic source of trunk fibroblasts as well as the axial skeleton, and local signals likely contribute to the diversification of distinct fibroblast subtypes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping