PUBLICATION
Impaired fin regeneration and angiogenesis in aged zebrafish and turquoise killifish
- Authors
- Örling, J., Kosonen, K., Villman, J., Reichard, M., Paatero, I.
- ID
- ZDB-PUB-230317-34
- Date
- 2023
- Source
- Biology Open 12(4): (Journal)
- Registered Authors
- Paatero, Ilkka
- Keywords
- Aging, Angiogenesis, Danio rerio, Fin, Nothobranchius furzeri, Regeneration, Turquoise killifish, VEGF, Wound healing, Zebrafish
- MeSH Terms
-
- Aged
- Animals
- Fundulidae*
- Humans
- Mammals/metabolism
- Vascular Endothelial Growth Factor A/metabolism
- Wound Healing
- Zebrafish*/metabolism
- Zebrafish Proteins
- PubMed
- 36919760 Full text @ Biol. Open
Citation
Örling, J., Kosonen, K., Villman, J., Reichard, M., Paatero, I. (2023) Impaired fin regeneration and angiogenesis in aged zebrafish and turquoise killifish. Biology Open. 12(4):.
Abstract
Impaired wound healing is associated with aging and has significant effects on human health on an individual level, but also the whole health care sector. Deficient angiogenesis appears to be involved in the process, but the underlying biology is still poorly understood. This is at least partially being explained by complexity and costs in using mammalian aging models. To understand aging-related vascular biology of impaired wound healing, we have utilized zebrafish and turquoise killifish fin regeneration models. The regeneration of caudal fin after resection was significantly reduced in old individuals in both species. Age-related changes in angiogenesis, vascular density and expression levels of angiogenesis biomarker VEGF-A were observed. Furthermore, anti-angiogenic drug, vascular endothelial growth factor receptor blocking inhibitor SU5416 reduced regeneration indicating a key role for angiogenesis in the regeneration of aging caudal fin despite aging-related changes in vasculature. Taken together, our data indicates that these fish fin regeneration models are suitable for studying aging-related decline in wound healing and associated alterations in aging vasculature.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping