PUBLICATION
Zebrafish pigment cells develop directly from persistent highly multipotent progenitors
- Authors
- Subkhankulova, T., Camargo Sosa, K., Uroshlev, L.A., Nikaido, M., Shriever, N., Kasianov, A.S., Yang, X., Rodrigues, F.S.L.M., Carney, T.J., Bavister, G., Schwetlick, H., Dawes, J.H.P., Rocco, A., Makeev, V.J., Kelsh, R.N.
- ID
- ZDB-PUB-230307-46
- Date
- 2023
- Source
- Nature communications 14: 12581258 (Journal)
- Registered Authors
- Carney, Tom, Kelsh, Robert
- Keywords
- none
- Datasets
- GEO:GSE185592
- MeSH Terms
-
- Animals
- Automobile Driving*
- Cell Differentiation/genetics
- Hematopoietic Stem Cells
- Multipotent Stem Cells
- Zebrafish*/genetics
- PubMed
- 36878908 Full text @ Nat. Commun.
Citation
Subkhankulova, T., Camargo Sosa, K., Uroshlev, L.A., Nikaido, M., Shriever, N., Kasianov, A.S., Yang, X., Rodrigues, F.S.L.M., Carney, T.J., Bavister, G., Schwetlick, H., Dawes, J.H.P., Rocco, A., Makeev, V.J., Kelsh, R.N. (2023) Zebrafish pigment cells develop directly from persistent highly multipotent progenitors. Nature communications. 14:12581258.
Abstract
Neural crest cells are highly multipotent stem cells, but it remains unclear how their fate restriction to specific fates occurs. The direct fate restriction model hypothesises that migrating cells maintain full multipotency, whilst progressive fate restriction envisages fully multipotent cells transitioning to partially-restricted intermediates before committing to individual fates. Using zebrafish pigment cell development as a model, we show applying NanoString hybridization single cell transcriptional profiling and RNAscope in situ hybridization that neural crest cells retain broad multipotency throughout migration and even in post-migratory cells in vivo, with no evidence for partially-restricted intermediates. We find that leukocyte tyrosine kinase early expression marks a multipotent stage, with signalling driving iridophore differentiation through repression of fate-specific transcription factors for other fates. We reconcile the direct and progressive fate restriction models by proposing that pigment cell development occurs directly, but dynamically, from a highly multipotent state, consistent with our recently-proposed Cyclical Fate Restriction model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping