PUBLICATION
Impact of the Voltage-Gated Calcium Channel Antagonist Nimodipine on the Development of Oligodendrocyte Precursor Cells
- Authors
- Enders, M., Weier, A., Chunder, R., An, Y., Bremm, F., Feigenspan, A., Buettner, C., Ekici, A.B., Mingardo, E., Odermatt, B., Kuerten, S.
- ID
- ZDB-PUB-230226-63
- Date
- 2023
- Source
- International Journal of Molecular Sciences 24(4): (Journal)
- Registered Authors
- Odermatt, Benjamin
- Keywords
- MS, OPC, Oli-Neu, dihydropyridines, myelination, neuroprotection, nimodipine, zebrafish
- MeSH Terms
-
- Animals
- Calcium Channel Blockers/pharmacology
- Calcium Channels, L-Type/metabolism
- Cell Differentiation
- Encephalomyelitis, Autoimmune, Experimental*/metabolism
- Mice
- MicroRNAs*/metabolism
- Multiple Sclerosis*/metabolism
- Myelin Sheath/metabolism
- Nimodipine/pharmacology
- Oligodendrocyte Precursor Cells*/metabolism
- Oligodendroglia/metabolism
- Zebrafish/genetics
- PubMed
- 36835129 Full text @ Int. J. Mol. Sci.
Citation
Enders, M., Weier, A., Chunder, R., An, Y., Bremm, F., Feigenspan, A., Buettner, C., Ekici, A.B., Mingardo, E., Odermatt, B., Kuerten, S. (2023) Impact of the Voltage-Gated Calcium Channel Antagonist Nimodipine on the Development of Oligodendrocyte Precursor Cells. International Journal of Molecular Sciences. 24(4):.
Abstract
Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). While most of the current treatment strategies focus on immune cell regulation, except for the drug siponimod, there is no therapeutic intervention that primarily aims at neuroprotection and remyelination. Recently, nimodipine showed a beneficial and remyelinating effect in experimental autoimmune encephalomyelitis (EAE), a mouse model of MS. Nimodipine also positively affected astrocytes, neurons, and mature oligodendrocytes. Here we investigated the effects of nimodipine, an L-type voltage-gated calcium channel antagonist, on the expression profile of myelin genes and proteins in the oligodendrocyte precursor cell (OPC) line Oli-Neu and in primary OPCs. Our data indicate that nimodipine does not have any effect on myelin-related gene and protein expression. Furthermore, nimodipine treatment did not result in any morphological changes in these cells. However, RNA sequencing and bioinformatic analyses identified potential micro (mi)RNA that could support myelination after nimodipine treatment compared to a dimethyl sulfoxide (DMSO) control. Additionally, we treated zebrafish with nimodipine and observed a significant increase in the number of mature oligodendrocytes (* p≤ 0.05). Taken together, nimodipine seems to have different positive effects on OPCs and mature oligodendrocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping