PUBLICATION
Microarray transcriptome datasets of maternal-zygotic DNA methyltransferase 3aa-/- zebrafish during early developmental stages
- Authors
- Shirai, M., Shimoda, N., Takahashi, H., Takayama, K., Kikuchi, Y.
- ID
- ZDB-PUB-230224-41
- Date
- 2023
- Source
- Data in brief 47: 108967108967 (Journal)
- Registered Authors
- Kikuchi, Yutaka
- Keywords
- Development, Dnmt3aa, Epigenetics, Microarray, Zebrafish
- MeSH Terms
- none
- PubMed
- 36820127 Full text @ Data Brief
Citation
Shirai, M., Shimoda, N., Takahashi, H., Takayama, K., Kikuchi, Y. (2023) Microarray transcriptome datasets of maternal-zygotic DNA methyltransferase 3aa-/- zebrafish during early developmental stages. Data in brief. 47:108967108967.
Abstract
DNA methylation is an epigenetic regulator mediated by DNA methyltransferases (Dnmts). The methylation is involved in control of gene expression in vertebrates. It has been reported that there are mainly two types of de novo Dnmts, Dnmt3a and Dnmt3b, in mammals. These two Dnmts function in DNA methylation in the distinct or overlapping genomic regions. The zebrafish homologs of mammalian Dnmt3a are Dnmt3aa and Dnmt3ab. We generated a maternal-zygotic dnmt3aa deficient mutant (MZdnmt3aa) to identify the specific target regions for DNA methylation in the zebrafish genome and their function in the developmental process. Microarray analysis revealed alterations in gene expression by knock-out of dnmt3aa in early zebrafish development. Microarray datasets were produced from samples at five different developmental stages: 1-2 cell, shield, 5-somite, 1-day post fertilization (dpf), and 2 dpf. Herein, we present novel raw and processed transcriptome datasets generated by analysis of the MZdnmt3aa-/- mutant. The raw microarray data are available through the Gene Expression Omnibus (GEO), accession number GSE202646. These transcriptome data may be useful for comparing differences in gene expression among species of Dnmt3a mutants and for analyzing human diseases caused by DNMT3A such as acute myelogenous leukemia (AML).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping