PUBLICATION
Social behavioral profiling by unsupervised deep learning reveals a stimulative effect of dopamine D3 agonists on zebrafish sociality
- Authors
- Geng, Y., Yates, C., Peterson, R.T.
- ID
- ZDB-PUB-230224-30
- Date
- 2023
- Source
- Cell reports methods 3: 100381100381 (Journal)
- Registered Authors
- Peterson, Randall
- Keywords
- ZeChat, behavioral classification, behavioral profiling, chemical screen, dopamine D3 agonists, piribedil, pramipexole, social stimulative, unsupervised deep learning, zebrafish
- MeSH Terms
-
- Animals
- Deep Learning*
- Dopamine
- Dopamine Agonists*/pharmacology
- Rats
- Rats, Sprague-Dawley
- Social Behavior
- Zebrafish
- PubMed
- 36814839 Full text @ Cell Rep Methods
Citation
Geng, Y., Yates, C., Peterson, R.T. (2023) Social behavioral profiling by unsupervised deep learning reveals a stimulative effect of dopamine D3 agonists on zebrafish sociality. Cell reports methods. 3:100381100381.
Abstract
It has been a major challenge to systematically evaluate and compare how pharmacological perturbations influence social behavioral outcomes. Although some pharmacological agents are known to alter social behavior, precise description and quantification of such effects have proven difficult. We developed a scalable social behavioral assay for zebrafish named ZeChat based on unsupervised deep learning to characterize sociality at high resolution. High-dimensional and dynamic social behavioral phenotypes are automatically classified using this method. By screening a neuroactive compound library, we found that different classes of chemicals evoke distinct patterns of social behavioral fingerprints. By examining these patterns, we discovered that dopamine D3 agonists possess a social stimulative effect on zebrafish. The D3 agonists pramipexole, piribedil, and 7-hydroxy-DPAT-HBr rescued social deficits in a valproic-acid-induced zebrafish autism model. The ZeChat platform provides a promising approach for dissecting the pharmacology of social behavior and discovering novel social-modulatory compounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping