PUBLICATION
Evaluation of the Antiseizure Activity of Endemic Plant Halfordia kendack Guillaumin and Its Main Constituent, Halfordin, on a Zebrafish Pentylenetetrazole (PTZ)-Induced Seizure Model
- Authors
- Skiba, A., Kozioł, E., Luca, S.V., Budzyńska, B., Podlasz, P., Van Der Ent, W., Shojaeinia, E., Esguerra, C.V., Nour, M., Marcourt, L., Wolfender, J.L., Skalicka-Woźniak, K.
- ID
- ZDB-PUB-230212-35
- Date
- 2023
- Source
- International Journal of Molecular Sciences 24(3): (Journal)
- Registered Authors
- Esguerra, Camila V., Podlasz, Piotr, Shojaeinia, Elham, van der Ent, Wietske
- Keywords
- LFP, coumarins, electrophysiology, epilepsy, liquid-liquid chromatography, rutaceae, zebrafish
- MeSH Terms
-
- Animals
- Anticonvulsants/toxicity
- Disease Models, Animal
- Epilepsy*/drug therapy
- Pentylenetetrazole*/pharmacology
- Seizures/chemically induced
- Seizures/drug therapy
- Seizures/metabolism
- Zebrafish
- PubMed
- 36768918 Full text @ Int. J. Mol. Sci.
Citation
Skiba, A., Kozioł, E., Luca, S.V., Budzyńska, B., Podlasz, P., Van Der Ent, W., Shojaeinia, E., Esguerra, C.V., Nour, M., Marcourt, L., Wolfender, J.L., Skalicka-Woźniak, K. (2023) Evaluation of the Antiseizure Activity of Endemic Plant Halfordia kendack Guillaumin and Its Main Constituent, Halfordin, on a Zebrafish Pentylenetetrazole (PTZ)-Induced Seizure Model. International Journal of Molecular Sciences. 24(3):.
Abstract
Epilepsy is a neurological disease that burdens over 50 million people worldwide. Despite the considerable number of available antiseizure medications, it is estimated that around 30% of patients still do not respond to available treatment. Herbal medicines represent a promising source of new antiseizure drugs. This study aimed to identify new drug lead candidates with antiseizure activity from endemic plants of New Caledonia. The crude methanolic leaf extract of Halfordia kendack Guillaumin (Rutaceae) significantly decreased (75 μg/mL and 100 μg/mL) seizure-like behaviour compared to sodium valproate in a zebrafish pentylenetetrazole (PTZ)-induced acute seizure model. The main coumarin compound, halfordin, was subsequently isolated by liquid-liquid chromatography and subjected to locomotor, local field potential (LFP), and gene expression assays. Halfordin (20 μM) significantly decreased convulsive-like behaviour in the locomotor and LFP analysis (by 41.4% and 60%, respectively) and significantly modulated galn, and penka gene expression.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping