PUBLICATION
Healing Wounds Efficiently with Biomimetic Soft Matter: Injectable Self-Healing Neutral Glycol Chitosan/Dibenzaldehyde-Terminated Poly(ethylene glycol) Hydrogel with Inherent Antibacterial Properties
- Authors
- Balitaan, J.N.I., Luo, W.J., Su, Y.W., Yu, C.Y., Wu, T.Y., Chang, C.A., Jia, H.W., Lin, S.R., Hsiao, C.D., Yeh, J.M.
- ID
- ZDB-PUB-230210-33
- Date
- 2023
- Source
- ACS applied bio materials 6(2): 552-565 (Journal)
- Registered Authors
- Hsiao, Chung-Der
- Keywords
- Schiff base, glycol chitosan, self-healing, wound healing, zebrafish
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents/chemistry
- Anti-Bacterial Agents/pharmacology
- Anti-Bacterial Agents/therapeutic use
- Biocompatible Materials/pharmacology
- Biomimetics
- Escherichia coli*
- Hydrogels/chemistry
- Polyethylene Glycols/chemistry
- Staphylococcus aureus*
- Wound Healing
- Zebrafish
- PubMed
- 36759183 Full text @ ACS Appl Bio Mater
Citation
Balitaan, J.N.I., Luo, W.J., Su, Y.W., Yu, C.Y., Wu, T.Y., Chang, C.A., Jia, H.W., Lin, S.R., Hsiao, C.D., Yeh, J.M. (2023) Healing Wounds Efficiently with Biomimetic Soft Matter: Injectable Self-Healing Neutral Glycol Chitosan/Dibenzaldehyde-Terminated Poly(ethylene glycol) Hydrogel with Inherent Antibacterial Properties. ACS applied bio materials. 6(2):552-565.
Abstract
The high prevalence of acquiring skin wounds, along with the emergence of antibiotic-resistant strains that lead to infections, impose a threat to the physical, mental, and socioeconomic health of society. Among the wide array of wound dressings developed, hydrogels are regarded as a biomimetic soft matter of choice owing to their ability to provide a moist environment ideal for healing. Herein, neutral glycol chitosan (GC) was cross-linked via imine bonds with varying concentrations of dibenzaldehyde-terminated polyethylene glycol (DP) to give glycol chitosan/dibenzaldehyde-terminated polyethylene glycol hydrogels (GC/DP). These dynamic Schiff base linkages (absorption peak at 1638 cm-1) within the hydrogel structure endowed their ability to recover from damage as characterized by high-low strain exposure in continuous step strain rheology. Along with their good injectability and biodegradability, the hydrogels exhibited remarkable inhibition against E. coli, P. aeruginosa, and S. aureus. GC/DP hydrogels demonstrated high LC50 values in vivo using zebrafish embryos as a model system due to their relative biocompatibility and a remarkable 93.4 ± 0.88% wound contraction at 30-dpw against 49.1 ± 3.40% of the control. To the best of our knowledge, this is the first study that developed injectable glycol chitosan/dibenzaldehyde-terminated polyethylene glycol self-healing hydrogels for application in wound healing with intrinsic bacteriostatic properties against the three bacteria.
Genes / Markers
Expression
Phenotype
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Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping