PUBLICATION
Intraocular delivery of ZIF-90-RhB-GW2580 nanoparticles prevents the progression of photoreceptor degeneration
- Authors
- Cao, P., Cheng, Y., Li, Z., Cheng, Y.J., Chu, X., Geng, C., Yin, X., Li, Y.
- ID
- ZDB-PUB-230208-15
- Date
- 2023
- Source
- Journal of nanobiotechnology 21: 4444 (Journal)
- Registered Authors
- Li, Yuhao
- Keywords
- Degeneration, GW2580, Intraocular delivery, Microglia, Photoreceptor, ZIF-90
- MeSH Terms
-
- Animals
- Nanoparticles*
- Retinal Degeneration*/drug therapy
- Retinal Degeneration*/prevention & control
- Zebrafish
- Zeolites*
- PubMed
- 36747224 Full text @ J Nanobiotechnology
Citation
Cao, P., Cheng, Y., Li, Z., Cheng, Y.J., Chu, X., Geng, C., Yin, X., Li, Y. (2023) Intraocular delivery of ZIF-90-RhB-GW2580 nanoparticles prevents the progression of photoreceptor degeneration. Journal of nanobiotechnology. 21:4444.
Abstract
Photoreceptor degeneration is one of the major causes of progressive blindness which lacks of curative treatment. GW2580, a highly selective inhibitor of colony-stimulating factor 1 receptor, has the protective potential on neurons; however, little was known about the application of GW2580 on photoreceptor degeneration. In this study, BV-2 and 661W cells coculture system was constructed to investigate the interaction between microglia and photoreceptors. GW2580 was loaded into zeolitic imidazolate framework-90-rhodamine B (ZIF-90-RhB) to synthesize a novel kind of nanoparticles, namely, ZIF-90-RhB-GW2580, through a one-step self-assembly approach. A photoreceptor degeneration model was generated by intense light exposure in zebrafish and ZIF-90-RhB-GW2580 nanoparticles were delivered by the intraocular injection. The results showed that in vitro GW2580 treatment promoted phenotypic transformation in microglia and led to the blockade of photoreceptor apoptosis. Following the intraocular delivery of ZIF-90-RhB-GW2580 nanoparticles, the microglial proliferation and inflammatory response were significantly inhibited; moreover, the photoreceptors underwent alleviated injury with a recovery of retinal structure and visual function. In conclusion, the intraocular injection of ZIF-90-RhB-GW2580 at the early stage enables the precise delivery and sustained release of the GW2580, thus preventing the progression of photoreceptor degeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping