PUBLICATION
Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish
- Authors
- Zhu, X., Xie, D., Zhu, Q., Li, Y., Cui, C.
- ID
- ZDB-PUB-230124-4
- Date
- 2023
- Source
- Frontiers in nutrition 9: 11007181100718 (Journal)
- Registered Authors
- Keywords
- behavior, serotonin, tryptophan peptides, zebrafish, β-lactoglobulin
- MeSH Terms
- none
- PubMed
- 36687713 Full text @ Front Nutr
Citation
Zhu, X., Xie, D., Zhu, Q., Li, Y., Cui, C. (2023) Preparation of β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in Zebrafish. Frontiers in nutrition. 9:11007181100718.
Abstract
This study aimed to obtain three Trp-containing peptides from β-lactoglobulin and study their effects on anxiety-like behaviors in zebrafish. Three Trp-containing peptides were prepared from β-lactoglobulin by selective enzymatic hydrolysis and identified by UPLC-Q-TOF MS/MS. The anxiety-like behaviors of zebrafish were reduced after two weeks of administrated of β-lactoglobulin Trp peptides (LAWP), VAGTWY, VAGTW and G TW(concentration of 56 μg/mL or 500 μg/mL). As an index of serotonergic activity, we assessed the enhancing abilities of 5-HT synthesis. The treatment remarkably enhanced the 5-HT synthesis by upregulation of Trp concentration and Trp hydroxylase activation. In addition, this study further validated the anti-anxiety effects of whey protein hydrolysate with a high Trp index in animal and the experimental results were consistent with those reported in previous studies. Our results showed that β-lactoglobulin Trp peptides ingestion has a significant anti-anxiety effect as evidenced by the increasing Trp concentration, TPH activation and 5-HT level compared to the control group, with the VAGTW being the more effective.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping