PUBLICATION

Cilia function as calcium-mediated mechanosensors that instruct left-right asymmetry

Authors
Djenoune, L., Mahamdeh, M., Truong, T.V., Nguyen, C.T., Fraser, S.E., Brueckner, M., Howard, J., Yuan, S.
ID
ZDB-PUB-230106-4
Date
2023
Source
Science (New York, N.Y.)   379: 717871-78 (Journal)
Registered Authors
Fraser, Scott E., Yuan, Shiaulou
Keywords
none
MeSH Terms
  • Calcium*/metabolism
  • Body Patterning*
  • Cilia*/physiology
  • Animals
  • Calcium Signaling*
  • TRPP Cation Channels/metabolism
  • Zebrafish*/growth & development
  • Zebrafish Proteins/metabolism
(all 8)
PubMed
36603098 Full text @ Science
Abstract
The breaking of bilateral symmetry in most vertebrates is critically dependent upon the motile cilia of the embryonic left-right organizer (LRO), which generate a directional fluid flow; however, it remains unclear how this flow is sensed. Here, we demonstrated that immotile LRO cilia are mechanosensors for shear force using a methodological pipeline that combines optical tweezers, light sheet microscopy, and deep learning to permit in vivo analyses in zebrafish. Mechanical manipulation of immotile LRO cilia activated intraciliary calcium transients that required the cation channel Polycystin-2. Furthermore, mechanical force applied to LRO cilia was sufficient to rescue and reverse cardiac situs in zebrafish that lack motile cilia. Thus, LRO cilia are mechanosensitive cellular levers that convert biomechanical forces into calcium signals to instruct left-right asymmetry.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hi4166TgTransgenic Insertion
1 - 1 of 1
Show
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
cfap298MO2-cfap298MRPHLNO
pkd2MO3-pkd2MRPHLNO
1 - 2 of 2
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Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Djenoune, L., Mahamdeh, M., Truong, T.V., Nguyen, C.T., Fraser, S.E., Brueckner, M., Howard, J., Yuan, S. (2023) Cilia function as calcium-mediated mechanosensors that instruct left-right asymmetry. Science (New York, N.Y.). 379:717871-78.