PUBLICATION
Cilia function as calcium-mediated mechanosensors that instruct left-right asymmetry
- Authors
- Djenoune, L., Mahamdeh, M., Truong, T.V., Nguyen, C.T., Fraser, S.E., Brueckner, M., Howard, J., Yuan, S.
- ID
- ZDB-PUB-230106-4
- Date
- 2023
- Source
- Science (New York, N.Y.) 379: 717871-78 (Journal)
- Registered Authors
- Fraser, Scott E., Yuan, Shiaulou
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning*
- Calcium*/metabolism
- Calcium Signaling*
- Cilia*/physiology
- TRPP Cation Channels/metabolism
- Zebrafish*/growth & development
- Zebrafish Proteins/metabolism
- PubMed
- 36603098 Full text @ Science
Citation
Djenoune, L., Mahamdeh, M., Truong, T.V., Nguyen, C.T., Fraser, S.E., Brueckner, M., Howard, J., Yuan, S. (2023) Cilia function as calcium-mediated mechanosensors that instruct left-right asymmetry. Science (New York, N.Y.). 379:717871-78.
Abstract
The breaking of bilateral symmetry in most vertebrates is critically dependent upon the motile cilia of the embryonic left-right organizer (LRO), which generate a directional fluid flow; however, it remains unclear how this flow is sensed. Here, we demonstrated that immotile LRO cilia are mechanosensors for shear force using a methodological pipeline that combines optical tweezers, light sheet microscopy, and deep learning to permit in vivo analyses in zebrafish. Mechanical manipulation of immotile LRO cilia activated intraciliary calcium transients that required the cation channel Polycystin-2. Furthermore, mechanical force applied to LRO cilia was sufficient to rescue and reverse cardiac situs in zebrafish that lack motile cilia. Thus, LRO cilia are mechanosensitive cellular levers that convert biomechanical forces into calcium signals to instruct left-right asymmetry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping