PUBLICATION
Utilizing a Population-Genetic Framework to Test for Gene-Environment Interactions between Zebrafish Behavior and Chemical Exposure
- Authors
- Thunga, P., Truong, L., Rericha, Y., Du, J., Morshead, M., Tanguay, R.L., Reif, D.M.
- ID
- ZDB-PUB-221223-9
- Date
- 2022
- Source
- Toxics 10(12): (Journal)
- Registered Authors
- Tanguay, Robyn L.
- Keywords
- chemical risk-assessment, gene-environment interaction, toxicity screening, zebrafish behavior
- MeSH Terms
- none
- PubMed
- 36548602 Full text @ Toxics
Citation
Thunga, P., Truong, L., Rericha, Y., Du, J., Morshead, M., Tanguay, R.L., Reif, D.M. (2022) Utilizing a Population-Genetic Framework to Test for Gene-Environment Interactions between Zebrafish Behavior and Chemical Exposure. Toxics. 10(12):.
Abstract
Individuals within genetically diverse populations display broad susceptibility differences upon chemical exposures. Understanding the role of gene-environment interactions (GxE) in differential susceptibility to an expanding exposome is key to protecting public health. However, a chemical's potential to elicit GxE is often not considered during risk assessment. Previously, we've leveraged high-throughput zebrafish (Danio rerio) morphology screening data to reveal patterns of potential GxE effects. Here, using a population genetics framework, we apportioned variation in larval behavior and gene expression in three different PFHxA environments via mixed-effect modeling to assess significance of GxE term. We estimated the intraclass correlation (ICC) between full siblings from different families using one-way random-effects model. We found a significant GxE effect upon PFHxA exposure in larval behavior, and the ICC of behavioral responses in the PFHxA exposed population at the lower concentration was 43.7%, while that of the control population was 14.6%. Considering global gene expression data, a total of 3746 genes showed statistically significant GxE. By showing evidence that heritable genetics are directly affecting gene expression and behavioral susceptibility of individuals to PFHxA exposure, we demonstrate how standing genetic variation in a heterogeneous population such as ours can be leveraged to test for potential GxE.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping