PUBLICATION
Receptor subunit compositions underly distinct potencies of a muscle relaxant in fast and slow muscle fibers
- Authors
- Yamashita, M., Egashira, Y., Nakamura, S., Sakata, S., Ono, F.
- ID
- ZDB-PUB-221029-3
- Date
- 2022
- Source
- Frontiers in Physiology 13: 1026646 (Journal)
- Registered Authors
- Ono, Fumihito
- Keywords
- acetylcholine receptor (AChR), muscle relaxant, muscle type, neuromuscular junction, zebrafish
- MeSH Terms
- none
- PubMed
- 36304584 Full text @ Front. Physiol.
Citation
Yamashita, M., Egashira, Y., Nakamura, S., Sakata, S., Ono, F. (2022) Receptor subunit compositions underly distinct potencies of a muscle relaxant in fast and slow muscle fibers. Frontiers in Physiology. 13:1026646.
Abstract
A line of studies in the 1960s-1980s suggested that muscle relaxants do not work uniformly on all skeletal muscles, though its mechanism has not been clarified. We showed here that a classical non-depolarizing muscle relaxant pancuronium inhibits fast muscle fibers at lower concentration compared to slow muscle fibers in zebrafish. The difference of effective concentration was observed in locomotion caused by tactile stimulation as well as in synaptic currents of the neuromuscular junction induced by motor neuron excitation. We further showed that this difference arises from the different composition of acetylcholine receptors between slow and fast muscle fibers in the neuromuscular junction of zebrafish. It will be interesting to examine the difference of subunit composition and sensitivity to muscle relaxants in other species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping