PUBLICATION
Pals1a and aPKCλ are not essential for Schwann cell migration, division or myelination in zebrafish
- Authors
- Boueid, M.J., Mikdache, A., Lesport, E., Delespierre, B., Loisel-Duwattez, J., Degerny, C., Tawk, M.
- ID
- ZDB-PUB-221027-3
- Date
- 2022
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 252(1): 145-155 (Journal)
- Registered Authors
- Boueid, Marie-Jose, Degerny, Cindy, Delespierre, Brigitte, Lesport, Emilie, Tawk, Marcel
- Keywords
- Schwann cell, aPKC, myelin, pals1, peripheral nervous system, polarity, zebrafish
- MeSH Terms
-
- Animals
- Axons/metabolism
- Cell Movement/physiology
- Myelin Sheath*/metabolism
- Neurogenesis
- Schwann Cells
- Zebrafish*
- PubMed
- 36284447 Full text @ Dev. Dyn.
Citation
Boueid, M.J., Mikdache, A., Lesport, E., Delespierre, B., Loisel-Duwattez, J., Degerny, C., Tawk, M. (2022) Pals1a and aPKCλ are not essential for Schwann cell migration, division or myelination in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 252(1):145-155.
Abstract
Background Schwann cells are specialized glial cells of the peripheral nervous system that produce myelin and promote fast action potential propagation. In order to myelinate, Schwann cells engage in a series of events that include migration and division along axons, followed by extensive cytoskeletal rearrangements that ensure axonal ensheathment and myelination. Schwann cells are polarized and extend their processes along an abaxonal-adaxonal axis. Here, we investigate the role of the apical polarity proteins, Pals1a and aPKCλ, in Schwann cell behavior during zebrafish development.
Results We analyzed zebrafish nok and has mutants deficient for pals1a and aPKCλ function respectively. Using live imaging, transmission electron microscopy and whole mount immunostaining, we show that Schwann cells can migrate and divide appropriately, exhibit normal radial sorting, express myelin markers and ensheath axons on time in has and nok mutants.
Conclusions Pals1a and aPKCλ are not essential for Schwann cell migration, division or myelination in zebrafish. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping