PUBLICATION
Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish
- Authors
- Abd Elmagid, L., Mittal, N., Bakis, I., Lien, C.L., Harrison, M.R.
- ID
- ZDB-PUB-221026-7
- Date
- 2022
- Source
- Journal of visualized experiments : JoVE (187): (Journal)
- Registered Authors
- Harrison, Michael, Lien, Ching-Ling (Ellen)
- Keywords
- none
- MeSH Terms
-
- Animals
- Heart
- Hydrogels/metabolism
- Lymphatic Vessels*/metabolism
- Myocardium/metabolism
- Zebrafish*
- PubMed
- 36282694 Full text @ J. Vis. Exp.
Citation
Abd Elmagid, L., Mittal, N., Bakis, I., Lien, C.L., Harrison, M.R. (2022) Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish. Journal of visualized experiments : JoVE. (187):.
Abstract
Zebrafish have proved to be an important model for studying cardiovascular formation and function during postembryonic development and regeneration. The present protocol describes a method for injecting fluorescent tracers into the zebrafish myocardium to study interstitial fluid and debris uptake into cardiac lymphatic vessels. To do so, microspheres (200 nm diameter) and quantum dots (<10 nm diameter) are introduced into the myocardium of live zebrafish, which can be tracked using ex vivo confocal microscopy. These tracers are then tracked intermittently over several hours to follow clearance from the myocardium into cardiac lymphatic vessels. Quantum dots are transported through cardiac lymphatic vessels away from the heart, while larger microspheres remain at the injection site for over three weeks. This method of intramyocardial injection can be extended to other uses, including the injection of encapsulated MS or hydrogels to locally release cells, proteins, or compounds of interest to a targeted region of the heart.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping