PUBLICATION
Whole-brain optical access in a small adult vertebrate with two- and three-photon microscopy
- Authors
- Akbari, N., Tatarsky, R.L., Kolkman, K.E., Fetcho, J.R., Bass, A.H., Xu, C.
- ID
- ZDB-PUB-221018-93
- Date
- 2022
- Source
- iScience 25: 105191 (Journal)
- Registered Authors
- Fetcho, Joseph R.
- Keywords
- Biological sciences, Biological sciences research methodologies, Biotechnology
- MeSH Terms
- none
- PubMed
- 36248737 Full text @ iScience
Citation
Akbari, N., Tatarsky, R.L., Kolkman, K.E., Fetcho, J.R., Bass, A.H., Xu, C. (2022) Whole-brain optical access in a small adult vertebrate with two- and three-photon microscopy. iScience. 25:105191.
Abstract
Although optical microscopy has allowed scientists to study the entire brain in early developmental stages, access to the brains of live, adult vertebrates has been limited. Danionella, a genus of miniature, transparent fish closely related to zebrafish has been introduced as a neuroscience model to study the adult vertebrate brain. However, the extent of optically accessible depth in these animals has not been quantitatively characterized. Here, we show that both two- and three-photon microscopy can access the entire depth and rostral-caudal extent of the adult wildtype Danionella dracula brain without any modifications to the animal other than mechanical stabilization. Three-photon microscopy provides higher signal-to-background ratio and optical sectioning of fluorescently labeled vasculature through the deepest part of the brain, the hypothalamus. Hence, we use multiphoton microscopy to penetrate the entire adult brain within the geometry of this genus' head structures and without the need for pigment removal.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping