PUBLICATION
Aerobic glycolysis is important for zebrafish larval wound closure and tail regeneration
- Authors
- Scott, C.A., Carney, T.J., Amaya, E.
- ID
- ZDB-PUB-220924-33
- Date
- 2022
- Source
- Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 30(6): 665-680 (Journal)
- Registered Authors
- Amaya, Enrique, Carney, Tom
- Keywords
- Laconic, Warburg Effect, appendage regeneration, lactate, metabolism, wound healing
- MeSH Terms
-
- Animals
- Glycolysis
- Humans
- Lactates/pharmacology
- Larva
- Regeneration/physiology
- Wound Healing*/physiology
- Zebrafish*/physiology
- PubMed
- 36148505 Full text @ Wound Repair Regen.
Citation
Scott, C.A., Carney, T.J., Amaya, E. (2022) Aerobic glycolysis is important for zebrafish larval wound closure and tail regeneration. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. 30(6):665-680.
Abstract
The underlying mechanisms of appendage regeneration remain largely unknown and uncovering these mechanisms in capable organisms has far-reaching implications for potential treatments in humans. Recent studies implicate a requirement for metabolic reprogramming reminiscent of the Warburg effect during successful appendage and organ regeneration. As changes are thus predicted to be highly dynamic, methods permitting direct, real-time visualization of metabolites at the tissue and organismal level, would offer a significant advance in defining the influence of metabolism on regeneration and healing. We sought to examine whether glycolytic activity was altered during larval fin regeneration, utilising the genetically encoded biosensor, Laconic, enabling the spatiotemporal assessment of lactate levels in living zebrafish. We present evidence for a rapid increase in lactate levels within minutes following injury, with a role of aerobic glycolysis in actomyosin contraction and wound closure. We also find a second wave of lactate production, associated with overall larval tail regeneration. Chemical inhibition of glycolysis attenuates both contraction of the wound and regrowth of tissue following tail amputation, suggesting aerobic glycolysis is necessary at two distinct stages of regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping