PUBLICATION

Nodal signaling regulates asymmetric cellular behaviors, driving clockwise rotation of the heart tube in zebrafish

Authors
Kidokoro, H., Saijoh, Y., Schoenwolf, G.C.
ID
ZDB-PUB-220923-1
Date
2022
Source
Communications biology   5: 996 (Journal)
Registered Authors
Kidokoro, Hinako
Keywords
none
MeSH Terms
  • Heart/physiology
  • Rotation
  • Myocardium*
  • Zebrafish Proteins
  • Animals
  • Zebrafish*
(all 6)
PubMed
36131094 Full text @ Commun Biol
Abstract
Clockwise rotation of the primitive heart tube, a process regulated by restricted left-sided Nodal signaling, is the first morphological manifestation of left-right asymmetry. How Nodal regulates cell behaviors to drive asymmetric morphogenesis remains poorly understood. Here, using high-resolution live imaging of zebrafish embryos, we simultaneously visualized cellular dynamics underlying early heart morphogenesis and resulting changes in tissue shape, to identify two key cell behaviors: cell rearrangement and cell shape change, which convert initially flat heart primordia into a tube through convergent extension. Interestingly, left cells were more active in these behaviors than right cells, driving more rapid convergence of the left primordium, and thereby rotating the heart tube. Loss of Nodal signaling abolished the asymmetric cell behaviors as well as the asymmetric convergence of the left and right heart primordia. Collectively, our results demonstrate that Nodal signaling regulates the magnitude of morphological changes by acting on basic cellular behaviors underlying heart tube formation, driving asymmetric deformation and rotation of the heart tube.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ncv536TgTransgenic Insertion
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    s1pr2MO1-s1pr2MRPHLNO
    spawMO2-spawMRPHLNO
    1 - 2 of 2
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    Fish
    No data available
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    Orthology
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    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
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    Citation
    Kidokoro, H., Saijoh, Y., Schoenwolf, G.C. (2022) Nodal signaling regulates asymmetric cellular behaviors, driving clockwise rotation of the heart tube in zebrafish. Communications biology. 5:996.