PUBLICATION
Establishment of a Zebrafish Xenograft Model for in Vivo Investigation of Nasopharyngeal Carcinoma
- Authors
- Huang, E., Huang, H., Wu, L., Li, B., He, Z., Zhang, J.
- ID
- ZDB-PUB-220907-9
- Date
- 2022
- Source
- Cell transplantation 31: 9636897221116085 (Journal)
- Registered Authors
- Zhang, Jingjing
- Keywords
- ATF7, Pin1, nasopharyngeal carcinoma, xenograft, zebrafish
- MeSH Terms
-
- Animals
- Carcinoma*/drug therapy
- Carcinoma*/genetics
- Carcinoma*/pathology
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Disease Models, Animal
- Gene Expression Regulation, Neoplastic
- Heterografts
- Humans
- NIMA-Interacting Peptidylprolyl Isomerase/metabolism
- Nasopharyngeal Carcinoma
- Nasopharyngeal Neoplasms*/drug therapy
- Zebrafish/metabolism
- PubMed
- 36062473 Full text @ Cell Transplant
Citation
Huang, E., Huang, H., Wu, L., Li, B., He, Z., Zhang, J. (2022) Establishment of a Zebrafish Xenograft Model for in Vivo Investigation of Nasopharyngeal Carcinoma. Cell transplantation. 31:9636897221116085.
Abstract
Nasopharyngeal carcinoma (NPC) is a unique malignant tumor of the head and neck. Despite higher survival rates by the combination of radiotherapy and chemotherapy, the recurrence or metastasis of NPC still occurs at about 10%. Therefore, there is urgent demand to develop more effective in vivo models for preclinical trials to investigate the mechanisms of NPC development and progression and to explore better treatment approaches. In this study, we transplanted human NPC CNE1 cells into zebrafish embryos to establish a xenograft model of NPC, where the proliferation and invasion behaviors of NPC cells were investigated in vivo. Combining in vitro and in vivo analyses, we found that activating transcription factor 7 (ATF7) was involved in the occurrence and development of NPC regulated by peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1). The zebrafish NPC xenograft model established here thereby provides an in vivo tool for exploring the occurrence and development of NPC, which may help to identify new tumor markers and develop new therapeutic strategies for the treatment of NPC.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping