PUBLICATION
Netrins and netrin receptors are essential for normal targeting of sensory axons in the zebrafish olfactory bulb
- Authors
- Dang, P., Barnes, D.T., Cheng, R.P., Xu, A., Ji Moon, Y., Sripad Kodukula, S., Raper, J.A.
- ID
- ZDB-PUB-220809-15
- Date
- 2022
- Source
- Neuroscience 508: 19-29 (Journal)
- Registered Authors
- Raper, Jonathan
- Keywords
- Axon Guidance, Olfactory, Olfactory Sensory Neuron, Protoglomerulus, Zebrafish
- MeSH Terms
-
- Animals
- Axons/metabolism
- Netrin Receptors/metabolism
- Netrin-1/metabolism
- Netrins/metabolism
- Olfactory Bulb/metabolism
- Olfactory Receptor Neurons*/physiology
- Receptors, Odorant*/metabolism
- Zebrafish/metabolism
- PubMed
- 35940453 Full text @ Neuroscience
Citation
Dang, P., Barnes, D.T., Cheng, R.P., Xu, A., Ji Moon, Y., Sripad Kodukula, S., Raper, J.A. (2022) Netrins and netrin receptors are essential for normal targeting of sensory axons in the zebrafish olfactory bulb. Neuroscience. 508:19-29.
Abstract
Olfactory sensory neurons that express related odorant receptors specifically target large identifiable neuropils called protoglomeruli when they first reach the olfactory bulb in the zebrafish. This crude odorant receptor-related mapping is further refined as odorant receptor-specific glomeruli segregate from protoglomeruli later in development. Netrins are a prominent class of axon guidance molecules whose contribution to olfactory circuit formation is poorly studied. Morpholino knock down experiments have suggested that Netrin/Dcc signaling is involved in normal protoglomerular targeting. Here we extend these findings with more detailed characterization and modeling of netrin expression, and by examining protoglomerular targeting in mutant lines fornetrin1a (ntn1a), netrin1b (ntn1b), and their receptorsunc5b,dcc, andneo1a. We confirm thatntn1a,ntn1b, anddccare required for normal protoglomerular guidance of a subset of olfactory sensory neurons that are labeled with the Tg(or111-7:IRES:Gal4) transgene. We also observe errors in the targeting of these axons inunc5bmutants, but not inneo1a mutants. Our findings are consistent with ntn1a andntn1bacting primarily as attractants for olfactory sensory neurons targeting the central zone protoglomerulus.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping