PUBLICATION
Timely Schwann cell division drives peripheral myelination in vivo via Laminin/cAMP pathway
- Authors
- Mikdache, A., Boueid, M.J., Lesport, E., Delespierre, B., Loisel-Duwattez, J., Degerny, C., Tawk, M.
- ID
- ZDB-PUB-220809-10
- Date
- 2022
- Source
- Development (Cambridge, England) 149(17): (Journal)
- Registered Authors
- Boueid, Marie-Jose, Degerny, Cindy, Delespierre, Brigitte, Lesport, Emilie, Tawk, Marcel
- Keywords
- Laminin/cAMP, MCPH, Mitotic spindle, Myelin, Schwann cells, Zebrafish
- MeSH Terms
-
- Axons/metabolism
- Cell Division/genetics
- Cells, Cultured
- Laminin*/genetics
- Laminin*/metabolism
- Myelin Sheath/metabolism
- Schwann Cells*/metabolism
- PubMed
- 35938454 Full text @ Development
Citation
Mikdache, A., Boueid, M.J., Lesport, E., Delespierre, B., Loisel-Duwattez, J., Degerny, C., Tawk, M. (2022) Timely Schwann cell division drives peripheral myelination in vivo via Laminin/cAMP pathway. Development (Cambridge, England). 149(17).
Abstract
Schwann cells (SC) migrate along peripheral axons and divide intensively to generate the right number of cells prior to axonal ensheathment; however, little is known regarding the temporal and molecular control of their division and its impact on myelination. We report that Sil, a spindle pole protein associated with autosomal recessive primary microcephaly (MCPH), is required for temporal mitotic exit of SC. In sil-deficient cassiopeia (csp-/-) mutants, SC fail to radially sort and myelinate peripheral axons. Elevation of cAMP, but not Rac1 activity in csp-/- restores myelin ensheathment. Most importantly, we show a significant decrease in Laminin expression within csp-/- posterior lateral line nerve and that forcing Laminin2 expression in csp-/- fully restores SC ability to myelinate. Thus, we unravel a novel and essential role for timely SC division in mediating Laminin expression to orchestrate radial sorting and peripheral myelination in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping