PUBLICATION

Origin and function of activated fibroblast states during zebrafish heart regeneration

Authors
Hu, B., Lelek, S., Spanjaard, B., El-Sammak, H., Simões, M.G., Mintcheva, J., Aliee, H., Schäfer, R., Meyer, A.M., Theis, F., Stainier, D.Y.R., Panáková, D., Junker, J.P.
ID
ZDB-PUB-220723-3
Date
2022
Source
Nature Genetics   54(8): 1227-1237 (Journal)
Registered Authors
Meyer, Alexander, Panáková, Daniela, Stainier, Didier
Keywords
none
Datasets
GEO:GSE158919, GEO:GSE159032
MeSH Terms
  • Animals
  • Fibroblasts
  • Myocytes, Cardiac/physiology
  • Cell Proliferation
  • Heart/physiology
  • Regeneration/genetics
  • Zebrafish Proteins*/genetics
  • Zebrafish*/genetics
(all 8)
PubMed
35864193 Full text @ Nat. Genet.
Abstract
The adult zebrafish heart has a high capacity for regeneration following injury. However, the composition of the regenerative niche has remained largely elusive. Here, we dissected the diversity of activated cell states in the regenerating zebrafish heart based on single-cell transcriptomics and spatiotemporal analysis. We observed the emergence of several transient cell states with fibroblast characteristics following injury, and we outlined the proregenerative function of collagen-12-expressing fibroblasts. To understand the cascade of events leading to heart regeneration, we determined the origin of these cell states by high-throughput lineage tracing. We found that activated fibroblasts were derived from two separate sources: the epicardium and the endocardium. Mechanistically, we determined Wnt signalling as a regulator of the endocardial fibroblast response. In summary, our work identifies specialized activated fibroblast cell states that contribute to heart regeneration, thereby opening up possible approaches to modulating the regenerative capacity of the vertebrate heart.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a131TgTransgenic Insertion
    c264TgTransgenic Insertion
      cn9TgTransgenic Insertion
        cz1701TgTransgenic Insertion
          hu5333TgTransgenic Insertion
            md80TgTransgenic Insertion
              pd42TgTransgenic Insertion
                zh701TgTransgenic Insertion
                  1 - 8 of 8
                  Show
                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  No data available
                  Antibodies
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  CeruleanEFGCerulean
                  CreEFGCre
                  EGFPEFGEGFP
                  GAL4EFGGAL4
                  mCherryEFGmCherry
                  NTREFGNTR
                  RFPEFGRFP
                  TomatoEFGTomato
                  VenusEFGVenus
                  YFPEFGYFP
                  1 - 10 of 10
                  Show
                  Mapping
                  No data available
                  Your Input Welcome
                  We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
                  Citation
                  Hu, B., Lelek, S., Spanjaard, B., El-Sammak, H., Simões, M.G., Mintcheva, J., Aliee, H., Schäfer, R., Meyer, A.M., Theis, F., Stainier, D.Y.R., Panáková, D., Junker, J.P. (2022) Origin and function of activated fibroblast states during zebrafish heart regeneration. Nature Genetics. 54(8):1227-1237.