PUBLICATION

Salviae miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection Protects against Myocardial Ischemia-Reperfusion Injury and Heart Failure

Authors

Kong, S., Zhou, D., Fan, Q., Zhao, T., Ren, C., Nie, H.

ID

ZDB-PUB-220712-10

Date

2022

Source

Computational and Mathematical Methods in Medicine 2022: 7809485 (Journal)

Registered Authors

Keywords

none

MeSH Terms

Apoptosis
Hypoxia/metabolism
Glucose
Rats
Zebrafish/metabolism
Signal Transduction
Myocardial Reperfusion Injury*/drug therapy
Myocardial Reperfusion Injury*/metabolism
Myocardial Reperfusion Injury*/prevention & control
Heart Failure*/drug therapy
Heart Failure*/etiology
Heart Failure*/prevention & control
MicroRNAs*/metabolism
Animals
Myocytes, Cardiac

(all 15)

PubMed

35813430 Full text @ Comput. Math. Methods Med.

Abstract

Purpose Myocardial ischemia-reperfusion (MIR) injury is a common stimulus for cardiac diseases like cardiac arrhythmias and heart failure and may cause high mortality rates. Salviae miltiorrhizae liguspyragine hydrochloride and glucose injection (SGI) has been widely used to treat myocardial and cerebral infarctions in China even though its pharmacological mechanisms are not completely clear.

Methods The protective effect and mechanism of SGI on MIR injury and heart failure were investigated through the H9c2 cell model induced by hypoxia/reoxygenation (H/R) and rapamycin, zebrafish model induced by H/R and isoprenaline, and rat MIR model.

Results SGI significantly reduced the infarct size and alleviated the impairment of cardiac functions in the MIR rat model and H/R zebrafish model and promoted cell viability of cardiomyocyte-like H9c2 cells under H/R condition. Consistently, SGI significantly downregulated the serum level of biomarkers for cardiac damage and attenuated the oxidative damage in the MIR and H/R models. We also found that SGI could downregulate the increased autophagy level in those MIR and H/R models since autophagy can contribute to the injurious effects of ischemia-reperfusion in the heart, suggesting that SGI may alleviate MIR injury via regulating the autophagy pathway. In addition, we demonstrated that SGI also played a protective role in the isoproterenol-induced zebrafish heart failure model, and SGI significantly downregulated the increased autophagy and SP1/GATA4 pathways.

Conclusion SGI may exert anti-MIR and heart failure by inhibiting activated autophagy and the SP1/GATA4 pathway.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment by environment: isoprenaline	Day 4	heart contraction decreased rate, abnormal	Figure 3
WT	chemical treatment by environment: isoprenaline	Day 4	heart decreased functionality, abnormal	Figure 3
WT	chemical treatment by environment: isoprenaline	Day 4	pericardium edematous, abnormal	Figure 3
WT	chemical treatment by environment: isoprenaline	Day 4	pericardium increased size, abnormal	Figure 3
WT	chemical treatment by environment: isoprenaline	Day 4	whole organism adrb1 expression increased amount, abnormal	Figure 4
WT	chemical treatment by environment: isoprenaline	Day 4	whole organism atg5 expression increased amount, abnormal	Figure 4
WT	chemical treatment by environment: isoprenaline	Day 4	whole organism atg7 expression increased amount, abnormal	Figure 4
WT	chemical treatment by environment: isoprenaline	Day 4	whole organism becn1 expression increased amount, abnormal	Figure 4
WT	chemical treatment by environment: isoprenaline	Day 4	whole organism gata4 expression increased amount, abnormal	Figure 4
WT	chemical treatment by environment: isoprenaline	Day 4	whole organism map1lc3b expression increased amount, abnormal	Figure 4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
twu34Tg	Tg(myl7:EGFP)	Transgenic Insertion
uwm8Tg	Tg(mpx:EGFP-Hsa.UTRN)	Transgenic Insertion

Human Disease / Model

Human Disease	Fish	Conditions	Evidence
congestive heart failure	WT	chemical treatment by environment: isoprenaline	TAS
myocardial infarction	WT	hypoxia	TAS

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Kong et al., 2022 ZDB-PUB-220712-10 PMID:35813430

Salviae miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection Protects against Myocardial Ischemia-Reperfusion Injury and Heart Failure

Kong et al., 2022

ZDB-PUB-220712-10
PMID:35813430