PUBLICATION
Computational refocusing of Jones matrix polarization-sensitive optical coherence tomography and investigation of defocus-induced polarization artifacts
- Authors
- Zhu, L., Makita, S., Oida, D., Miyazawa, A., Oikawa, K., Mukherjee, P., Lichtenegger, A., Distel, M., Yasuno, Y.
- ID
- ZDB-PUB-220702-2
- Date
- 2022
- Source
- Biomedical Optics Express 13: 2975-2994 (Journal)
- Registered Authors
- Distel, Martin
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 35774308 Full text @ Biomed. Opt. Express
Citation
Zhu, L., Makita, S., Oida, D., Miyazawa, A., Oikawa, K., Mukherjee, P., Lichtenegger, A., Distel, M., Yasuno, Y. (2022) Computational refocusing of Jones matrix polarization-sensitive optical coherence tomography and investigation of defocus-induced polarization artifacts. Biomedical Optics Express. 13:2975-2994.
Abstract
Here we demonstrate a long-depth-of-focus imaging method using polarization sensitive optical coherence tomography (PS-OCT). This method involves a combination of Fresnel-diffraction-model-based phase sensitive computational refocusing and Jones-matrix based PS-OCT (JM-OCT). JM-OCT measures four complex OCT images corresponding to four polarization channels. These OCT images are computationally refocused as preserving the mutual phase consistency. This method is validated using a static phantom, postmortem zebrafish, and ex vivo porcine muscle samples. All the samples demonstrated successful computationally-refocused birefringence and degree-of-polarization-uniformity (DOPU) images. We found that defocusing induces polarization artifacts, i.e., incorrectly high birefringence values and low DOPU values, which are substantially mitigated by computational refocusing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
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