PUBLICATION
The Extracts of Polygonum cuspidatum Root and Rhizome Block the Entry of SARS-CoV-2 Wild-Type and Omicron Pseudotyped Viruses via Inhibition of the S-Protein and 3CL Protease
- Authors
- Lin, S., Wang, X., Tang, R.W., Lee, H.C., Chan, H.H., Choi, S.S.A., Dong, T.T., Leung, K.W., Webb, S.E., Miller, A.L., Tsim, K.W.
- ID
- ZDB-PUB-220625-22
- Date
- 2022
- Source
- Molecules 27(12): (Journal)
- Registered Authors
- Miller, Andrew L., Webb, Sarah E.
- Keywords
- Polygoni Cuspidati Rhizoma et Radix, SARS-CoV-2, omicron variant, pseudovirus entry, traditional Chinese medicine, zebrafish larvae
- MeSH Terms
-
- Animals
- Antiviral Agents/analysis
- Antiviral Agents/pharmacology
- COVID-19*/drug therapy
- Fallopia japonica*/chemistry
- Peptide Hydrolases
- Plant Extracts/analysis
- Plant Extracts/pharmacology
- Rhizome/chemistry
- SARS-CoV-2
- Viral Pseudotyping
- Zebrafish
- PubMed
- 35744929 Full text @ Molecules
Citation
Lin, S., Wang, X., Tang, R.W., Lee, H.C., Chan, H.H., Choi, S.S.A., Dong, T.T., Leung, K.W., Webb, S.E., Miller, A.L., Tsim, K.W. (2022) The Extracts of Polygonum cuspidatum Root and Rhizome Block the Entry of SARS-CoV-2 Wild-Type and Omicron Pseudotyped Viruses via Inhibition of the S-Protein and 3CL Protease. Molecules. 27(12).
Abstract
COVID-19, resulting from infection by the SARS-CoV-2 virus, caused a contagious pandemic. Even with the current vaccines, there is still an urgent need to develop effective pharmacological treatments against this deadly disease. Here, we show that the water and ethanol extracts of the root and rhizome of Polygonum cuspidatum (Polygoni Cuspidati Rhizoma et Radix), a common Chinese herbal medicine, blocked the entry of wild-type and the omicron variant of the SARS-CoV-2 pseudotyped virus into fibroblasts or zebrafish larvae, with IC50 values ranging from 0.015 to 0.04 mg/mL. The extracts were shown to inhibit various aspects of the pseudovirus entry, including the interaction between the spike protein (S-protein) and the angiotensin-converting enzyme II (ACE2) receptor, and the 3CL protease activity. Out of the chemical compounds tested in this report, gallic acid, a phytochemical in P. cuspidatum, was shown to have a significant anti-viral effect. Therefore, this might be responsible, at least in part, for the anti-viral efficacy of the herbal extract. Together, our data suggest that the extracts of P. cuspidatum inhibit the entry of wild-type and the omicron variant of SARS-CoV-2, and so they could be considered as potent treatments against COVID-19.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping