PUBLICATION
A Subset of Oligodendrocyte Lineage Cells Interact With the Developing Dorsal Root Entry Zone During Its Genesis
- Authors
- Green, L.A., Gallant, R.M., Brandt, J.P., Nichols, E.L., Smith, C.J.
- ID
- ZDB-PUB-220624-4
- Date
- 2022
- Source
- Frontiers in Cellular Neuroscience 16: 893629 (Journal)
- Registered Authors
- Keywords
- DRG, development, glia, oligodendrocyte, sensory neuron, zebrafish
- MeSH Terms
- none
- PubMed
- 35734217 Full text @ Front. Cell. Neurosci.
Citation
Green, L.A., Gallant, R.M., Brandt, J.P., Nichols, E.L., Smith, C.J. (2022) A Subset of Oligodendrocyte Lineage Cells Interact With the Developing Dorsal Root Entry Zone During Its Genesis. Frontiers in Cellular Neuroscience. 16:893629.
Abstract
Oligodendrocytes are the myelinating cell of the CNS and are critical for the functionality of the nervous system. In the packed CNS, we know distinct profiles of oligodendrocytes are present. Here, we used intravital imaging in zebrafish to identify a distinct oligodendrocyte lineage cell (OLC) that resides on the dorsal root ganglia sensory neurons in the spinal cord. Our profiling of OLC cellular dynamics revealed a distinct cell cluster that interacts with peripheral sensory neurons at the dorsal root entry zone (DREZ). With pharmacological, physical and genetic manipulations, we show that the entry of dorsal root ganglia pioneer axons across the DREZ is important to produce sensory located oligodendrocyte lineage cells. These oligodendrocyte lineage cells on peripherally derived sensory neurons display distinct processes that are stable and do not express mbpa. Upon their removal, sensory behavior related to the DRG neurons is abolished. Together, these data support the hypothesis that peripheral neurons at the DREZ can also impact oligodendrocyte development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping