PUBLICATION
Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus
- Authors
- Sun, X., Tao, B., Wang, Y., Hu, W., Sun, Y.
- ID
- ZDB-PUB-220611-6
- Date
- 2022
- Source
- International Journal of Molecular Sciences 23(11): (Journal)
- Registered Authors
- Hu, Wei, Sun, Xiaoyun
- Keywords
- germline stem cells, ovary, ovotestis, testis, the swamp eel, transplantation
- MeSH Terms
-
- Adult Stem Cells*
- Animals
- Female
- Germ Cells
- Gonads
- Male
- Sex Determination Processes
- Zebrafish*/genetics
- PubMed
- 35682541 Full text @ Int. J. Mol. Sci.
Citation
Sun, X., Tao, B., Wang, Y., Hu, W., Sun, Y. (2022) Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus. International Journal of Molecular Sciences. 23(11).
Abstract
Germline stem cells (GSCs) are a group of unique adult stem cells in gonads that act as important transmitters for genetic information. Donor GSCs have been used to produce offspring by transplantation in fisheries. In this study, we successfully isolated and enriched GSCs from the ovary, ovotestis, and testis of Monopterus albus, one of the most important breeding freshwater fishes in China. Transcriptome comparison assay suggests that a distinct molecular signature exists in each type of GSC, and that different signaling activities are required for the maintenance of distinct GSCs. Functional analysis shows that fGSCs can successfully colonize and contribute to the germline cell lineage of a host zebrafish gonad after transplantation. Finally, we describe a simple feeder-free method for the isolation and enrichment of GSCs that can contribute to the germline cell lineage of zebrafish embryos and generate the germline chimeras after transplantation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping