PUBLICATION
Short chain fatty acids (SCFAs) improves TNBS-induced colitis in zebrafish
- Authors
- Morales Fénero, C., Amaral, M.A., Xavier, I.K., Padovani, B.N., Paredes, L.C., Takiishi, T., Lopes-Ferreira, M., Lima, C., Colombo, A., Saraiva Câmara, N.O.
- ID
- ZDB-PUB-220503-8
- Date
- 2021
- Source
- Current research in immunology 2: 142-154 (Journal)
- Registered Authors
- Keywords
- Intestinal inflammation, Larval zebrafish, Microbiota, Short-chain fatty acids, TNBS
- MeSH Terms
- none
- PubMed
- 35492385 Full text @ Curr Res Immunol
Citation
Morales Fénero, C., Amaral, M.A., Xavier, I.K., Padovani, B.N., Paredes, L.C., Takiishi, T., Lopes-Ferreira, M., Lima, C., Colombo, A., Saraiva Câmara, N.O. (2021) Short chain fatty acids (SCFAs) improves TNBS-induced colitis in zebrafish. Current research in immunology. 2:142-154.
Abstract
The short-chain fatty acids (SCFAs) are metabolites originated from the fermentation of dietary fibers and amino acids produced by the bacteria of the intestinal microbiota. The most abundant SCFAs, acetate, propionate, and butyrate, have been proposed as a treatment for inflammatory bowel diseases (IBDs) due to their anti-inflammatory properties. This work aimed to analyze the effects of the treatment of three combined SCFAs in TNBS-induced intestinal inflammation in zebrafish larvae. Here, we demonstrated that SCFAs significantly increased the survival of TNBS-exposed larvae, preserved the intestinal endocytic function, reduced the expression of inflammatory cytokines and the intestinal recruitment of neutrophils caused by TNBS. However, SCFAs treatment did not appear to avoid TNBS-induced tissue damage in the intestinal wall and did not restore the number of mucus-producing goblet cells. Finally, exposure to TNBS induced dysbiosis of the microbiota with an increase in Betaproteobacteria and Actinobacteria, while the treatment with SCFAs maintained these population levels similar to control. Thus, we demonstrate that the treatment of three combined SCFAs presented anti-inflammatory properties previously seen in mammals, opening an opportunity to use zebrafish to explore the potential benefit of these and other metabolites to treat inflammation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping