PUBLICATION
LED Zappelin': An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging
- Authors
- Zimmermann, M.J.Y., Maia Chagas, A., Bartel, P., Pop, S., Prieto-Godino, L.L., Baden, T.
- ID
- ZDB-PUB-220503-19
- Date
- 2020
- Source
- HardwareX 8: e00127 (Journal)
- Registered Authors
- Baden, Tom
- Keywords
- 2 Photon, LED, Light stimulation, Open hardware, Optogenetics, Visual neuroscience
- MeSH Terms
- none
- PubMed
- 35498254 Full text @ HardwareX
Citation
Zimmermann, M.J.Y., Maia Chagas, A., Bartel, P., Pop, S., Prieto-Godino, L.L., Baden, T. (2020) LED Zappelin': An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging. HardwareX. 8:e00127.
Abstract
Two-photon (2P) microscopy is a cornerstone technique in neuroscience research. However, combining 2P imaging with spectrally arbitrary light stimulation can be challenging due to crosstalk between stimulation light and fluorescence detection. To overcome this limitation, we present a simple and low-cost electronic solution based on an ESP32 microcontroller and a TLC5947 LED driver to rapidly time-interleave stimulation and detection epochs during scans. Implemented for less than $100, our design can independently drive up to 24 arbitrary spectrum LEDs to meet user requirements. We demonstrate the utility of our stimulator for colour vision experiments on the in vivo tetrachromatic zebrafish retina and for optogenetic circuit mapping in Drosophila.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping