PUBLICATION
Zebrafish Heme Oxygenase 1a Is Necessary for Normal Development and Macrophage Migration
- Authors
- Luo, K., Ogawa, M., Ayer, A., Britton, W.J., Stocker, R., Kikuchi, K., Oehlers, S.H.
- ID
- ZDB-PUB-220203-9
- Date
- 2022
- Source
- Zebrafish 19(1): 7-17 (Journal)
- Registered Authors
- Kikuchi, Kazu, Luo, Kaiming, Oehlers, Stefan, Ogawa, Mashahito
- Keywords
- HMOX, heme oxygenase, macrophage
- MeSH Terms
-
- Animals
- Zebrafish*/metabolism
- Oxidative Stress
- Macrophages/metabolism
- Heme Oxygenase (Decyclizing)*/metabolism
- PubMed
- 35108124 Full text @ Zebrafish
Abstract
Heme oxygenase function is highly conserved between vertebrates where it plays important roles in normal embryonic development and controls oxidative stress. Expression of the zebrafish heme oxygenase 1 genes is known to be responsive to oxidative stress suggesting a conserved physiological function. In this study, we generate a knockout allele of zebrafish hmox1a and characterize the effects of hmox1a and hmox1b loss on embryonic development. We find that loss of hmox1a or hmox1b causes developmental defects in only a minority of embryos, in contrast to Hmox1 gene deletions in mice that cause loss of most embryos. Using a tail wound inflammation assay we find a conserved role for hmox1a, but not hmox1b, in normal macrophage migration to the wound site. Together our results indicate that zebrafish hmox1a has clearly a partitioned role from hmox1b that is more consistent with conserved functions of mammalian Heme oxygenase 1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping