PUBLICATION
Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model
- Authors
- Kuo, C.T., Lai, Y.S., Lu, S.R., Lee, H., Chang, H.H.
- ID
- ZDB-PUB-220126-4
- Date
- 2021
- Source
- Biology 11(1): (Journal)
- Registered Authors
- Lee, Hsinyu
- Keywords
- chemiluminescence, drug screening, microarray chip, zebrafish xenograft
- MeSH Terms
- none
- PubMed
- 35053002 Full text @ Biology (Basel)
Citation
Kuo, C.T., Lai, Y.S., Lu, S.R., Lee, H., Chang, H.H. (2021) Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model. Biology. 11(1):.
Abstract
Purpose The aim of this study was to develop a rapid and automatic drug screening platform using microcrater-arrayed (µCA) cell chips.
Methods The µCA chip was fabricated using a laser direct writing technique. The fabrication time required for one 9 × 9 microarray wax chip was as quick as 1 min. On a nanodroplet handling platform, the chip was pre-coated with anti-cancer drugs, including cyclophosphamide, cisplatin, doxorubicin, oncovin, etoposide, and 5-fluorouracil, and their associated mixtures. Cell droplets containing 100 SK-N-DZ or MCF-7 cells were then loaded onto the chip. Cell viability was examined directly through a chemiluminescence assay on the chip using the CellTiter-Glo assay.
Results The time needed for the drug screening assay was demonstrated to be less than 30 s for a total of 81 tests. The prediction of optimal drug synergy from the µCA chip was found by matching it to that of the zebrafish MCF-7 tumor xenograft model, instead of the conventional 96-well plate assay. In addition, the critical reagent volume and cell number for each µCA chip test were 200 nL and 100 cells, respectively, which were significantly lower than 100 µL and 4000 cells, which were achieved using the 96-well assay.
Conclusion Our study for the µCA chip platform could improve the high-throughput drug synergy screening targeting the applications of tumor cell biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping