PUBLICATION
Estrogens regulate early embryonic development of the olfactory sensory system via estrogen-responsive glia
- Authors
- Takesono, A., Schirrmacher, P., Scott, A., Green, J.M., Lee, O., Winter, M.J., Kudoh, T., Tyler, C.R.
- ID
- ZDB-PUB-220114-6
- Date
- 2022
- Source
- Development (Cambridge, England) 149(1): (Journal)
- Registered Authors
- Kudoh, Tetsuhiro, Lee, Okhyun, Tyler, Charles R.
- Keywords
- Embryogenesis, Endocrine disrupting chemicals, Olfactory glomerular development, Olfactory-mediated behaviour, Radial glia progenitor cells, Transgenic zebrafish embryo
- MeSH Terms
-
- Animals
- Estrogen Receptor Antagonists/pharmacology
- Estrogens/metabolism*
- Fulvestrant/pharmacology
- Neurogenesis*
- Neuroglia/cytology*
- Neuroglia/drug effects
- Neuroglia/metabolism
- Olfactory Bulb/cytology
- Olfactory Bulb/drug effects
- Olfactory Bulb/embryology*
- Olfactory Receptor Neurons/cytology
- Olfactory Receptor Neurons/metabolism
- Receptors, Estrogen/antagonists & inhibitors
- Synapses/metabolism
- Synapses/physiology
- Zebrafish
- PubMed
- 35023540 Full text @ Development
Citation
Takesono, A., Schirrmacher, P., Scott, A., Green, J.M., Lee, O., Winter, M.J., Kudoh, T., Tyler, C.R. (2022) Estrogens regulate early embryonic development of the olfactory sensory system via estrogen-responsive glia. Development (Cambridge, England). 149(1):.
Abstract
Estrogens are well-known to regulate development of sexual dimorphism of the brain; however, their role in embryonic brain development prior to sex-differentiation is unclear. Using estrogen biosensor zebrafish models, we found that estrogen activity in the embryonic brain occurs from early neurogenesis specifically in a type of glia in the olfactory bulb (OB), which we name estrogen-responsive olfactory bulb (EROB) cells. In response to estrogen, EROB cells overlay the outermost layer of the OB and interact tightly with olfactory sensory neurons at the olfactory glomeruli. Inhibiting estrogen activity using an estrogen receptor antagonist, ICI182,780 (ICI), and/or EROB cell ablation impedes olfactory glomerular development, including the topological organisation of olfactory glomeruli and inhibitory synaptogenesis in the OB. Furthermore, activation of estrogen signalling inhibits both intrinsic and olfaction-dependent neuronal activity in the OB, whereas ICI or EROB cell ablation results in the opposite effect on neuronal excitability. Altering the estrogen signalling disrupts olfaction-mediated behaviour in later larval stage. We propose that estrogens act on glia to regulate development of OB circuits, thereby modulating the local excitability in the OB and olfaction-mediated behaviour.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping