PUBLICATION
Tfap2b specifies an embryonic melanocyte stem cell that retains adult multifate potential
- Authors
- Brombin, A., Simpson, D.J., Travnickova, J., Brunsdon, H., Zeng, Z., Lu, Y., Young, A.I.J., Chandra, T., Patton, E.E.
- ID
- ZDB-PUB-220113-16
- Date
- 2022
- Source
- Cell Reports 38: 110234 (Journal)
- Registered Authors
- Brombin, Alessandro, Patton, E. Elizabeth
- Keywords
- ErbB, chemical genetics, fate mapping, melanocyte lineage, melanocyte stem cells, neural crest, scRNA-seq, tfap2b, zebrafish
- Datasets
- GEO:GSE178364
- MeSH Terms
-
- Animals
- Cell Differentiation/genetics
- Cell Lineage/genetics
- Melanocytes/metabolism*
- Melanocytes/physiology
- Pigmentation/genetics
- Skin/metabolism
- Skin Pigmentation/genetics
- Stem Cells/classification*
- Stem Cells/metabolism
- Transcription Factor AP-2/genetics
- Transcription Factor AP-2/metabolism*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 35021087 Full text @ Cell Rep.
Citation
Brombin, A., Simpson, D.J., Travnickova, J., Brunsdon, H., Zeng, Z., Lu, Y., Young, A.I.J., Chandra, T., Patton, E.E. (2022) Tfap2b specifies an embryonic melanocyte stem cell that retains adult multifate potential. Cell Reports. 38:110234.
Abstract
Melanocytes, the pigment-producing cells, are replenished from multiple stem cell niches in adult tissue. Although pigmentation traits are known risk factors for melanoma, we know little about melanocyte stem cell (McSC) populations other than hair follicle McSCs and lack key lineage markers with which to identify McSCs and study their function. Here we find that Tfap2b and a select set of target genes specify an McSC population at the dorsal root ganglia in zebrafish. Functionally, Tfap2b is required for only a few late-stage embryonic melanocytes, and is essential for McSC-dependent melanocyte regeneration. Fate mapping data reveal that tfap2b+ McSCs have multifate potential, and are the cells of origin for large patches of adult melanocytes, two other pigment cell types (iridophores and xanthophores), and nerve-associated cells. Hence, Tfap2b confers McSC identity in early development, distinguishing McSCs from other neural crest and pigment cell lineages, and retains multifate potential in the adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping