PUBLICATION
The landscape of regulatory genes in brain-wide neuronal phenotypes of a vertebrate brain
- Authors
- Zhang, H., Wang Haifang, H., Shen, X., Jia, X., Yu, S., Qiu, X., Wang, Y., Du, J., Yan, J., He, J.
- ID
- ZDB-PUB-211214-46
- Date
- 2021
- Source
- eLIFE 10: (Journal)
- Registered Authors
- Du, Jiu Lin, He, Jie, Jia, Xinling, Qiu, Xiaoying, Shen, Xiaoyu, Yu, Shuguang, Zhang, Hui
- Keywords
- neuroscience, zebrafish
- MeSH Terms
-
- Animals
- Brain/metabolism
- Gene Expression Profiling
- Gene Expression Regulation, Developmental*/genetics
- Genes, Regulator*
- Neurons/physiology*
- Phenotype*
- Transcriptome*
- Zebrafish/genetics*
- PubMed
- 34895465 Full text @ Elife
Citation
Zhang, H., Wang Haifang, H., Shen, X., Jia, X., Yu, S., Qiu, X., Wang, Y., Du, J., Yan, J., He, J. (2021) The landscape of regulatory genes in brain-wide neuronal phenotypes of a vertebrate brain. eLIFE. 10:.
Abstract
Multidimensional landscapes of regulatory genes in neuronal phenotypes at whole-brain levels in the vertebrate remain elusive. We generated single-cell transcriptomes of ~67,000 region- and glutamatergic/neuromodulator-identifiable cells from larval zebrafish brains. Hierarchical clustering based on effector gene profiles ('terminal features') distinguished major brain cell types. Sister clusters at hierarchical termini displayed similar terminal features. It was further verified by a population-level statistical method. Intriguingly, glutamatergic/GABAergic sister clusters mostly expressed distinct transcriptional factor (TF) profiles ('convergent pattern'), whereas neuromodulator-type sister clusters predominantly expressed the same TF profiles ('matched pattern'). Interestingly, glutamatergic/GABAergic clusters with similar TF profiles could also display different terminal features ('divergent pattern'). It led us to identify a library of RNA-binding proteins that differentially marked divergent pair clusters, suggesting the post-transcriptional regulation of neuron diversification. Thus, our findings reveal multidimensional landscapes of transcriptional and post-transcriptional regulators in whole-brain neuronal phenotypes in the zebrafish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping