PUBLICATION
In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
- Authors
- Chen, W., Natan, R.G., Yang, Y., Chou, S.W., Zhang, Q., Isacoff, E.Y., Ji, N.
- ID
- ZDB-PUB-211118-9
- Date
- 2021
- Source
- Nature communications 12: 6630 (Journal)
- Registered Authors
- Isacoff, Ehud
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism*
- Dendritic Spines/metabolism
- Glutamic Acid/metabolism*
- Imaging, Three-Dimensional/methods*
- Mice
- Microscopy, Fluorescence, Multiphoton
- Molecular Imaging
- Sensitivity and Specificity
- Synapses/metabolism*
- Visual Cortex/cytology
- Visual Cortex/diagnostic imaging
- Visual Cortex/metabolism
- Zebrafish
- PubMed
- 34785691 Full text @ Nat. Commun.
Citation
Chen, W., Natan, R.G., Yang, Y., Chou, S.W., Zhang, Q., Isacoff, E.Y., Ji, N. (2021) In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution. Nature communications. 12:6630.
Abstract
Studying neuronal activity at synapses requires high spatiotemporal resolution. For high spatial resolution in vivo imaging at depth, adaptive optics (AO) is required to correct sample-induced aberrations. To improve temporal resolution, Bessel focus has been combined with two-photon fluorescence microscopy (2PFM) for fast volumetric imaging at subcellular lateral resolution. To achieve both high-spatial and high-temporal resolution at depth, we develop an efficient AO method that corrects the distorted wavefront of Bessel focus at the objective focal plane and recovers diffraction-limited imaging performance. Applying AO Bessel focus scanning 2PFM to volumetric imaging of zebrafish larval and mouse brains down to 500 µm depth, we demonstrate substantial improvements in the sensitivity and resolution of structural and functional measurements of synapses in vivo. This enables volumetric measurements of synaptic calcium and glutamate activity at high accuracy, including the simultaneous recording of glutamate activity of apical and basal dendritic spines in the mouse cortex.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping