PUBLICATION

Proper Balance of Small GTPase rab10 Is Critical for PGC Migration in Zebrafish

Authors
Mo, C., Li, W., Jia, K., Liu, W., Yi, M.
ID
ZDB-PUB-211116-16
Date
2021
Source
International Journal of Molecular Sciences   22(21): (Journal)
Registered Authors
Liu, Wei, Yi, Meisheng
Keywords
miR-202-5p, migration, primordial germ cells, rab10
MeSH Terms
  • Animals
  • Cell Proliferation
  • Cell Movement*
  • Zebrafish
  • Germ Cells/cytology
  • Germ Cells/physiology*
  • Monomeric GTP-Binding Proteins/genetics
  • Monomeric GTP-Binding Proteins/metabolism*
  • MicroRNAs/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • rab GTP-Binding Proteins/genetics
  • rab GTP-Binding Proteins/metabolism*
(all 13)
PubMed
34769390 Full text @ Int. J. Mol. Sci.
Abstract
MicroRNAs (miRNAs) play important roles in post-transcriptional repression in nearly every biological process including germ cell development. Previously, we have identified a zebrafish germ plasm-specific miRNA miR-202-5p, which regulates PGC migration through targeting cdc42se1 to protect cdc42 expression. However, knockdown of cdc42se1 could not significantly rescue PGC migration in maternal miR-202 mutant (MmiR-202) embryos, indicating that there are other target genes of miR-202-5p required for the regulation of PGC migration. Herein, we revealed the transcriptional profiles of wild type and MmiR-202 PGCs and obtained 129 differentially expressed genes (DEGs), of which 42 DEGs were enriched cell migration-related signaling pathways. From these DEGs, we identified two novel miR-202-5p target genes prdm12b and rab10. Furthermore, we found that disruption of rab10 expression led to significantly migratory defects of PGC by overexpression of rab10 siRNA, or WT, inactive as well as active forms of rab10 mRNA, and WT rab10 overexpression mediated migratory defects could be partially but significantly rescued by overexpression of miR-202-5p, demonstrating that rab10 is an important factor involved miR-202-5p mediated regulation of PGC migration. Taken together, the present results provide significant information for understanding the molecular mechanism by which miR-202-5p regulates PGC migration in zebrafish.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ihb14TgTransgenic Insertion
    zf3308
      		Small Deletion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      No data available
      Antibodies
      No data available
      Orthology
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      1 - 1 of 1
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      Mapping
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      Citation
      Mo, C., Li, W., Jia, K., Liu, W., Yi, M. (2021) Proper Balance of Small GTPase rab10 Is Critical for PGC Migration in Zebrafish. International Journal of Molecular Sciences. 22(21):.