PUBLICATION

Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish

Authors
Safarian, N., Houshangi-Tabrizi, S., Zoidl, C., Zoidl, G.R.
ID
ZDB-PUB-211116-14
Date
2021
Source
International Journal of Molecular Sciences   22(21): (Journal)
Registered Authors
Safarian, Nickie, Zoidl, Christiane, Zoidl, Georg
Keywords
Muller glia, behavior, circadian clock, pannexin1b, retinal ganglion cells, transcriptomics, visual system, zebrafish
Datasets
GEO:GSE181853
MeSH Terms
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Swimming*
  • Motion Perception*
  • Vision, Ocular*
  • RNA-Seq
  • Connexins/genetics
  • Connexins/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Animals
(all 11)
PubMed
34769181 Full text @ Int. J. Mol. Sci.
Abstract
Pannexin1 (Panx1) can form ATP-permeable channels that play roles in the physiology of the visual system. In the zebrafish two ohnologs of Panx1, Panx1a and Panx1b, have unique and shared channel properties and tissue expression patterns. Panx1a channels are located in horizontal cells of the outer retina and modulate light decrement detection through an ATP/pH-dependent mechanisms and adenosine/dopamine signaling. Here, we decipher how the strategic localization of Panx1b channels in the inner retina and ganglion cell layer modulates visually evoked motor behavior. We describe a panx1b knockout model generated by TALEN technology. The RNA-seq analysis of 6 days post-fertilization larvae is confirmed by real-time PCR and paired with testing of locomotion behaviors by visual motor and optomotor response tests. We show that the loss of Panx1b channels disrupts the retinal response to an abrupt loss of illumination and it decreases the larval ability to follow leftward direction of locomotion in low light conditions. We concluded that the loss of Panx1b channels compromises the final output of luminance as well as motion detection. The Panx1b protein also emerges as a modulator of the circadian clock system. The disruption of the circadian clock system in mutants suggests that Panx1b could participate in non-image forming processes in the inner retina.
Genes / Markers
Marker Marker Type Name
bmal1aGENEbasic helix-loop-helix ARNT like 1a
bmal1bGENEbasic helix-loop-helix ARNT like 1b
bmal2GENEbasic helix-loop-helix ARNT like 2
cipcaGENECLOCK-interacting pacemaker a
clockaGENEclock circadian regulator a
clockbGENEclock circadian regulator b
cry1aGENEcryptochrome circadian regulator 1a
cry1bGENEcryptochrome circadian regulator 1b
cry2GENEcryptochrome circadian regulator 2
cry3bGENEcryptochrome circadian regulator 3b
1 - 10 of 21
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Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
yku2
    Small Deletion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    panx1bTALEN1-panx1bTALEN
    1 - 1 of 1
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    Fish
    1 - 2 of 2
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    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab2-panx1bpolyclonalIgGRabbit
    Ab12-gfappolyclonal
      Rabbit
      1 - 2 of 2
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      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available