PUBLICATION

De novo identification of mammalian ciliary motility proteins using cryo-EM

Authors
Gui, M., Farley, H., Anujan, P., Anderson, J.R., Maxwell, D.W., Whitchurch, J.B., Botsch, J.J., Qiu, T., Meleppattu, S., Singh, S.K., Zhang, Q., Thompson, J., Lucas, J.S., Bingle, C.D., Norris, D.P., Roy, S., Brown, A.
ID
ZDB-PUB-211030-14
Date
2021
Source
Cell   184(23): 5791-5806.e19 (Journal)
Registered Authors
Roy, Sudipto
Keywords
axonemal dyneins, ciliary motility, ciliopathies, cryo-EM, doublet microtubules, microtubule inner proteins
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cilia/metabolism
  • Cilia/ultrastructure*
  • Cryoelectron Microscopy*
  • Dyneins/metabolism
  • Embryo, Mammalian/metabolism
  • Female
  • Male
  • Mammals/metabolism*
  • Mice, Inbred C57BL
  • Microtubule Proteins/chemistry
  • Microtubules/metabolism
  • Microtubules/ultrastructure
  • Models, Molecular
  • Mutation/genetics
  • Proteins/metabolism*
  • Proteins/ultrastructure*
  • Trachea/anatomy & histology
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
34715025 Full text @ Cell
Abstract
Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine of cilia, the axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model of the 48-nm repeat of a mammalian DMT, derived from a cryoelectron microscopy (cryo-EM) map of the complex isolated from bovine respiratory cilia. The structure uncovers principles of doublet microtubule organization and features specific to vertebrate cilia, including previously unknown MIPs, a luminal bundle of tektin filaments, and a pentameric dynein-docking complex. We identify a mechanism for bridging 48- to 24-nm periodicity across the microtubule wall and show that loss of the proteins involved causes defective ciliary motility and laterality abnormalities in zebrafish and mice. Our structure identifies candidate genes for diagnosis of ciliopathies and provides a framework to understand their functions in driving ciliary motility.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping