PUBLICATION
DNA template strand segregation in developing zebrafish
- Authors
- Glasauer, S.M.K., Triemer, T., Neef, A.B., Neuhauss, S.C.F., Luedtke, N.W.
- ID
- ZDB-PUB-211001-12
- Date
- 2021
- Source
- Cell chemical biology 28(11): 1638-1647.e4 (Journal)
- Registered Authors
- Neuhauss, Stephan
- Keywords
- DNA metabolic labeling, azide-alkyne "click" cycloaddition, cell division, developmental biology, eye development, fluorescence microscopy, stem cells
- MeSH Terms
-
- Animals
- DNA/metabolism*
- Zebrafish/growth & development
- PubMed
- 34592171 Full text @ Cell Chem Biol
Citation
Glasauer, S.M.K., Triemer, T., Neef, A.B., Neuhauss, S.C.F., Luedtke, N.W. (2021) DNA template strand segregation in developing zebrafish. Cell chemical biology. 28(11):1638-1647.e4.
Abstract
Asymmetric inheritance of sister chromatids has long been predicted to be linked to discordant fates of daughter cells and even hypothesized to minimize accumulation of mutations in stem cells. Here, we use (2'S)-2'-deoxy-2'-fluoro-5-ethynyluridine (F-ara-EdU), bromodeoxyuridine (BrdU), and light sheet microscopy to track embryonic DNA in whole zebrafish. Larval development results in rapid depletion of older DNA template strands from stem cell niches in the retina, brain, and intestine. Prolonged label retention occurs in quiescent progenitors that resume replication in later development. High-resolution microscopy reveals no evidence of asymmetric template strand segregation in >100 daughter cell pairs, making it improbable that asymmetric DNA segregation prevents mutational burden according to the immortal strand hypothesis in developing zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping