PUBLICATION
Protective Effects and Mechanisms of Procyanidins on Parkinson's Disease In Vivo and In Vitro
- Authors
- Chen, J., Chen, Y., Zheng, Y., Zhao, J., Yu, H., Zhu, J., Li, D.
- ID
- ZDB-PUB-210929-18
- Date
- 2021
- Source
- Molecules 26(18): (Journal)
- Registered Authors
- Keywords
- Nrf2/ARE pathway, PC12 cells, Parkinson’s disease, procyanidins, zebrafish
- MeSH Terms
-
- Animals
- Antioxidants
- Oxidative Stress
- PC12 Cells
- Parkinson Disease*
- Proanthocyanidins*
- Rats
- PubMed
- 34577027 Full text @ Molecules
Citation
Chen, J., Chen, Y., Zheng, Y., Zhao, J., Yu, H., Zhu, J., Li, D. (2021) Protective Effects and Mechanisms of Procyanidins on Parkinson's Disease In Vivo and In Vitro. Molecules. 26(18):.
Abstract
This research assessed the molecular mechanism of procyanidins (PCs) against neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its metabolite 1-methyl-4-phenylpyridinium (MPP+) induced Parkinson's disease (PD) models. In vitro, PC12 cells were incubated with PCs or deprenyl for 24 h, and then exposed to 1.5 mM MPP+ for 24 h. In vivo, zebrafish larvae (AB strain) 3 days post-fertilization (dpf) were incubated with deprenyl or PCs in 400 μM MPTP for 4 days. Compared with MPP+/MPTP alone, PCs significantly improved antioxidant activities (e.g., glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT)), and decreased levels of reactive oxygen species (ROS) and malondialdehyde (MDA). Furthermore, PCs significantly increased nuclear Nrf2 accumulation in PC12 cells and raised the expression of NQO1, HO-1, GCLM, and GCLC in both PC12 cells and zebrafish compared to MPP+/MPTP alone. The current study shows that PCs have neuroprotective effects, activate the nuclear factor-erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway and alleviate oxidative damage in MPP+/MPTP-induced PD models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping