PUBLICATION
Genetic compensation between Pax3 and Pax7 in zebrafish appendicular muscle formation
- Authors
- Nord, H., Kahsay, A., Dennhag, N., Pedrosa Domellöf, F., von Hofsten, J.
- ID
- ZDB-PUB-210827-6
- Date
- 2021
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 251(9): 1423-1438 (Journal)
- Registered Authors
- Nord, Hanna, von Hofsten, Jonas
- Keywords
- appendicular myogenesis, limb development, muscle regeneration
- MeSH Terms
-
- Animals
- Mammals
- Muscle Development/genetics
- Muscle, Skeletal
- PAX3 Transcription Factor/genetics
- PAX7 Transcription Factor/genetics
- Paired Box Transcription Factors*/genetics
- Zebrafish*/genetics
- PubMed
- 34435397 Full text @ Dev. Dyn.
Citation
Nord, H., Kahsay, A., Dennhag, N., Pedrosa Domellöf, F., von Hofsten, J. (2021) Genetic compensation between Pax3 and Pax7 in zebrafish appendicular muscle formation. Developmental Dynamics : an official publication of the American Association of Anatomists. 251(9):1423-1438.
Abstract
Background Migrating muscle progenitors delaminate from the somite and subsequently form muscle tissue in distant anatomical regions such as the paired appendages, or limbs. In amniotes, this process requires a signaling cascade including the transcription factor paired box 3 (Pax3).
Results In this study we found that, unlike in mammals, pax3a/3b double mutant zebrafish develop near to normal appendicular muscle. By analyzing numerous mutant combinations of pax3a, pax3b and pax7a and pax7b, we determined that there is a feed-back system and a compensatory mechanism between Pax3 and Pax7 in this developmental process, even though Pax7 alone is not required for appendicular myogenesis. pax3a/3b/7a/7b quadruple mutant developed muscle-less pectoral fins.
Conclusions We found that Pax3 and Pax7 are redundantly required during appendicular myogenesis in zebrafish, where Pax7 is able to activate the same developmental programs as Pax3 in the pre-migratory progenitor cells. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping