PUBLICATION
Evaluation of the Antidiabetic Potential of Extracts of Urtica dioica, Apium graveolens, and Zingiber officinale in Mice, Zebrafish, and Pancreatic β-Cell
- Authors
- Pérez Gutiérrez, R.M., Muñiz-Ramirez, A., Garcia-Campoy, A.H., Mota Flores, J.M.
- ID
- ZDB-PUB-210811-3
- Date
- 2021
- Source
- Plants (Basel, Switzerland) 10(7): (Journal)
- Registered Authors
- Keywords
- Apium graveolens, Urtica dioica, Zingiber officinale, diabetes, mice, pancreatic β- RINm5F cell, zebrafish
- MeSH Terms
- none
- PubMed
- 34371645 Full text @ Plants (Basel)
Citation
Pérez Gutiérrez, R.M., Muñiz-Ramirez, A., Garcia-Campoy, A.H., Mota Flores, J.M. (2021) Evaluation of the Antidiabetic Potential of Extracts of Urtica dioica, Apium graveolens, and Zingiber officinale in Mice, Zebrafish, and Pancreatic β-Cell. Plants (Basel, Switzerland). 10(7):.
Abstract
Medicinal plants are commonly used in the treatment of diabetes, particularly as they contain flavonoids and phenolic compounds. The present study aims to investigate the activities of a polyherbal formulation made from Urtica dioica, Apium graveolens, and Zingiber officinale (UAZ) against streptozotocin-nicotinamide ((STZ-NA)-induced type 2 diabetes in CD1 mice, glucose-induced type 2 diabetes (T2DM) in zebrafish, and high glucose-induced damage in RINm5F pancreatic β-cells. In fasting mice, plasma glucose, glycosylated hemoglobin (HbA1C), lipid hydroperoxides (LOOH), thiobarbituric acid reactive substances (TBARS), and lipid profiles were significantly increased, whereas insulin, enzymatic antioxidants, and carbohydrate metabolic enzymes were altered significantly in diabetic mice. Zebrafish had similar glucose levels, liver enzymes, and lipid profiles compared to mice. The study investigated the effects of the extract in enhancing cell viability, insulin secretion, and reducing lipid peroxidation and intracellular reactive oxygen species (ROS) levels in RINm5F cells damaged by high glucose. All the above biochemical parameters were enhanced in both mice and zebrafish treated; the combined extract UAZ normalized all the biochemical parameters. The medicinal plant extracts, used either separately or in combination, ameliorated the adverse effect of glucose on cell viability and functionality of beta-RINm5F cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping