PUBLICATION
Cardiac toxicity assessment of pendimethalin in zebrafish embryos
- Authors
- Meng, Y., Zhong, K., Chen, S., Huang, Y., Wei, Y., Wu, J., Liu, J., Xu, Z., Guo, J., Liu, F., Lu, H.
- ID
- ZDB-PUB-210720-15
- Date
- 2021
- Source
- Ecotoxicology and environmental safety 222: 112514 (Journal)
- Registered Authors
- Lu, Huiqiang
- Keywords
- Cardiac toxicity assessment, Oxidative stress, Pendimethalin, Zebrafish
- MeSH Terms
-
- Aniline Compounds
- Animals
- Apoptosis
- Cardiotoxicity/metabolism
- Embryo, Nonmammalian*/metabolism
- Oxidative Stress
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 34280841 Full text @ Ecotoxicol. Environ. Saf.
- CTD
- 34280841
Citation
Meng, Y., Zhong, K., Chen, S., Huang, Y., Wei, Y., Wu, J., Liu, J., Xu, Z., Guo, J., Liu, F., Lu, H. (2021) Cardiac toxicity assessment of pendimethalin in zebrafish embryos. Ecotoxicology and environmental safety. 222:112514.
Abstract
Pendimethalin (PND) is one of the best sellers of selective herbicide in the world and has been frequently detected in the water. However, little is known about its effects on cardiac development. In this study, we used zebrafish to investigate the developmental and cardiac toxicity of PND. We exposed the zebrafish embryos with a serial of concentrations at 3, 4, and 5 mg/L at 5.5-72 h post-fertilization (hpf). We found that PND exposure can reduce the heart rate, survival rate, and body length of zebrafish embryos. Furthermore, we identified many malformations including pericardial and yolk sac edema, spinal deformity, and cardiac looping abnormality. In addition, PND increased the expression of reactive oxygen species and malondialdehyde and reduced the activity of superoxide dismutase (Antioxidant enzymes); We examined the expression of cardiac development-related genes and the apoptosis markers, and found changes of the following marker: vmhc, nppa, tbx5a, nkx2.5, gata4, tbx2b and FoxO1, bax, bcl-2, p53, casp-9, casp-3. Our data showed that activation of Wnt pathway can rescue the cardiac abnormalities caused by PND. Our results provided new evidence for the toxicity of PND and suggested that the PND residual should be treated as a hazard in the environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping