PUBLICATION
A transgenic system for targeted ablation of reproductive and maternal-effect genes
- Authors
- Bertho, S., Kaufman, O., Lee, K., Santos-Ledo, A., Dellal, D., Marlow, F.L.
- ID
- ZDB-PUB-210622-24
- Date
- 2021
- Source
- Development (Cambridge, England) 148(12): (Journal)
- Registered Authors
- Marlow, Florence
- Keywords
- Germ cells, Maternal effect, Mutagenesis
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryonic Development/genetics*
- Gene Expression
- Gene Knockdown Techniques*
- Gene Targeting*/methods
- Genetic Vectors/genetics
- Germ Cells/metabolism
- Humans
- Maternal Inheritance*
- Mutagenesis
- Reproducibility of Results
- Reproduction/genetics*
- Transgenes*
- PubMed
- 34143203 Full text @ Development
Citation
Bertho, S., Kaufman, O., Lee, K., Santos-Ledo, A., Dellal, D., Marlow, F.L. (2021) A transgenic system for targeted ablation of reproductive and maternal-effect genes. Development (Cambridge, England). 148(12):.
Abstract
Maternally provided gene products regulate the earliest events of embryonic life, including formation of the oocyte that will develop into an egg, and eventually into an embryo. Forward genetic screens have provided invaluable insights into the molecular regulation of embryonic development, including the essential contributions of some genes whose products must be provided to the transcriptionally silent early embryo for normal embryogenesis, called maternal-effect genes. However, other maternal-effect genes are not accessible due to their essential zygotic functions during embryonic development. Identifying these regulators is essential to fill the large gaps in our understanding of the mechanisms and molecular pathways contributing to fertility and to maternally regulated developmental processes. To identify these maternal factors, it is necessary to bypass the earlier requirement for these genes so that their potential later functions can be investigated. Here, we report reverse genetic systems to identify genes with essential roles in zebrafish reproductive and maternal-effect processes. As proof of principle and to assess the efficiency and robustness of mutagenesis, we used these transgenic systems to disrupt two genes with known maternal-effect functions: kif5ba and bucky ball.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping